Framing technology challenges associated with improving cancer immunotherapies
Thought leader Jim Heath introduces the Lab on a Chip Immunotherapy thematic collection.
Tumor-on-a-chip platforms to study cancer–immune system crosstalk in the era of immunotherapy
Immunotherapy is a powerful therapeutic approach able to re-educate the immune system to fight cancer. In this scenario, tumor-on-chip can help to choose the right immunotherapeutic treatment.
Critical Review
Recent advances in microfluidic technologies for cell-to-cell interaction studies
Microfluidic cell cultures are ideally positioned to become the next generation of in vitro diagnostic tools for biomedical research, where key biological processes such as cell signalling and dynamic cell-to-cell interactions can be reliably analysed under reproducible physiological cell culture conditions.
A multilayered blood vessel/tumor tissue chip to investigate T cell infiltration into solid tumor tissues
We developed a multilayered blood vessel/tumor tissue chip (MBTC) that allows systematic investigation on T cell tumor infiltration. Key characteristics of T cell dynamics in tumor microenvironments are recapitulated in the MBTCs.
Machine learning-aided quantification of antibody-based cancer immunotherapy by natural killer cells in microfluidic droplets
Comparative proteomic profiling and development of convolution neural network algorithm for quantifying discrete target interaction by engineered NK cells in microfluidic droplets.
Droplet encapsulation improves accuracy of immune cell cytokine capture assays
In-droplet cytokine capture assays combined with FACS to accurately identify and isolate activated immune cells.
MATE-Seq: microfluidic antigen-TCR engagement sequencing
Barcoded nanoparticles and an integrated DLD and droplet generator device are used to purify and sequence rare antigen-specific T cells.
Single cell arrays of hematological cancer cells for assessment of lymphocyte cytotoxicity dynamics, serial killing, and extracellular molecules
A new microwell assays to asses lymphocyte cytotoxicity against hematological cancer cells.
Microfluidic on-demand engineering of exosomes towards cancer immunotherapy
3D printing-based facile microfabrication of a microfluidic culture chip integrates harvesting, antigenic modification, and photo-release of surface engineered exosomes in one workflow, which enables rapid and real-time production of therapeutic exosomes for advancing cancer immunotherapy.
Functional TCR T cell screening using single-cell droplet microfluidics
Droplet-based single cell platform allows functional screening and sorting of desirable TCR T cells to accelerate development of adoptive T cell therapies.
Paper
3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade
Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy.
Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting
Our results demonstrate that multi-directional magnetic ratcheting offers a unique separation system for dealing with clinical samples that contain either rare cells or significantly small volumes.
A multiplexed microfluidic system for evaluation of dynamics of immune–tumor interactions
A system for perfusing and interacting tumor fragments and immune cells and testing drug response with image analytics is reported.
Cancer immunotherapy μ-environment LabChip: taking advantage of optoelectronic tweezers
An OET-based LabChip was developed to provide a stable and static culture μ-environment for cancer immunotherapy studies. The TiOPc-based OET facilitates the studies of cell–cell interaction resulting in apoptotic progress of cancer cells.
About this collection
A collection of recent reviews and research papers on the topic of immunotherapy and immunoengineering published in Lab on a Chip and curated by the Lab on a Chip Editorial Board.
This topic area is currently the focus of a thematic collection in this field, led by Thought leader James R. Heath. More details about how to submit to the collection can be found at rsc.li/immunotherapy-blog.