Biosynthesis and regulatory mechanisms of benzylisoquinoline alkaloids in medicinal plants

Lingyue Qin, Yanxia Liu, Qianliang Ming and Peng Li

M, 2025, 4, 0
DOI: 10.48130/mpb-0025-0025

Multi-Omics Roadmap to Plant-Derived Medicines

Jia-Yu Xue, Si-Jie Liu, Jing Wang, Xin-Cheng Huang, Zhi-Chao Xu, Xiao-Xue Fang, Zhen Li and Yves Van de Peer

Engineering, 2025
DOI: 10.1016/j.eng.2025.10.019

Functional divergence of NCS homologs in the PR10 family drives tissue-specific berberine accumulation in Coptis chinensis Franch

Mengyu Zhang, Yongjun Peng, Xinyue Zhu, Yating Zheng, Siyu Zhao, Rui Tan, Jiayu Zhou, Fengming Ren, Hai Liao and Hezhong Jiang

Plant Physiology and Biochemistry, 2025, 229, 110450
DOI: 10.1016/j.plaphy.2025.110450

Chelidonium majus L.: A Current Perspective on Isoquinoline Alkaloids, Emerging Phytochemicals, Alkaloid Biosynthesis, and Biological Activities

Ramona Romanu, Sergio Liga, Maria Roberta Tripon, Florin Huiban, Dan Iliescu, Cristina Adriana Dehelean and Tulcan Camelia

Plants, 2025, 14, 2627
DOI: 10.3390/plants14172627

Biosynthesis of benzylisoquinoline alkaloids and its evolution in plants

Ya Tian, Zhoujie An, Qi Li, Ge Bai, Qiyue Zhang, Yuzhuo Dong, Yong Chen, Shilin Chen, Zhichao Xu and Ranran Gao

Plant Communications, 2026, 7, 101786
DOI: 10.1016/j.xplc.2026.101786

CRISPR/Cas9 gene editing systems for enhancing secondary metabolite biosynthesis via reproductive tissue modification

Damanbhalang Rynjah, K. Sandhanam, Bedanta Bhattacharjee, Bhargab Deka, Arzoo Newar, Tutumoni Kalita, Junmoni Nath, Abdul Baquee Ahmed, Ram Kumar Sahu and Trishna Das

Discov. Plants, 2025, 2
DOI: 10.1007/s44372-025-00334-w

Fine-Tuned Transcription Factor Engineering and Precursor Rewiring Drive Enhanced Production of Berberine in Nicotiana benthamiana

Chenfei Tian, Qian Zhang, Yulian Zhang, Zhaotao Yan, Yuhan Wu, Haili Liu and Yong Wang

Engineering, 2025
DOI: 10.1016/j.eng.2025.10.021

The gap-free genome and functional characterization of O-methyltransferases provide insights into the biosynthesis of sanguinarine and chelerythrine in Eomecon chionantha

Peng Yang, Yuxin Qi, Yutong Cao, Xinlan Wang, Jiaxin Tan, Mingli Zhang, Yue Han and Xueshuang Huang

Plant Physiology and Biochemistry, 2026, 232, 111112
DOI: 10.1016/j.plaphy.2026.111112

Gene and genome duplications have contrasting impacts on biosynthetic and flower developmental pathways in California poppy

Le-Han Rössner, Clemens Rössner, Doudou Kong, Dominik Lotz, Andrea Weisert, Yasuyuki Yamada, Fumihiko Sato, Kevin Davies, Oliver Rupp, Jörg Fuchs, Ethan A Baldwin, John Lovell, Michael R McKain, Kerrie Barry, Tomas Bruna, Jayson Talag, Jerry Jenkins, Rachel Walstead, Jane Grimwood, Jeremy Schmutz, James H Leebens-Mack and Annette Becker

The Plant Cell, 2026, 38
DOI: 10.1093/plcell/koag039

Engineering Substrate Promiscuity and Catalytic Efficiency of Benzylisoquinoline Alkaloids N-Methyltransferases

Yuyang Zhang, Pan Yang, Zhenshan Chen, Chunsheng Tian, Yanni Zhao, Junhai Xiao and Wenli Li

J. Agric. Food Chem., 2026, 74, 8523
DOI: 10.1021/acs.jafc.5c16426

Hybrid genome and enzyme characterization uncover novel glycosyltransferases associated with benzylisoquinoline alkaloid chemodiversity in lotus

Hedi Zhao, Xinyi Qi, Yan Liu, Zhuyin Chen, Yifan Wang, Jing Que, Yanyan Su, Ling Yuan, Gangqiang Dong, An Liu, Zhennan Wang and Sha Chen

Journal of Advanced Research, 2026
DOI: 10.1016/j.jare.2026.04.032

Molecular Networking-Guided Discovery of Hypecoleptopines B–H, Seven Pairs of Racemic Spiro-benzylisoquinoline Alkaloids with Anti-Neuroinflammatory Activity from Hypecoum leptocarpum

Sheng Li, Yinling Wei, Qiang Zhang, Xinjian Zhang, Bodou Zhang, Jingwen Zhao, Xiao-Jiang Hao and Yu Zhang

J. Nat. Prod., 2025, 88, 2768
DOI: 10.1021/acs.jnatprod.5c01148

Quantitative determination of coptisine and berberine hydrochloride in Corydalis conspersa by high-performance liquid chromatography and quality evaluation

DU QING

Chinese Journal of Analytical Chemistry, 2025, 53, 100620
DOI: 10.1016/j.cjac.2025.100620

Genome-wide identification of O-methyltransferase genes in Stephania yunnanensis and their roles in benzylisoquinoline alkaloid biosynthesis

Can Wang, Hongying Peng, Baimei Liu, Guyin Lin, Yuan Pan, Xian mei Yin, Zhiyin Yu, Liang Leng and Shilin Chen

International Journal of Biological Macromolecules, 2026, 356, 151603
DOI: 10.1016/j.ijbiomac.2026.151603

Functional characterization and regioselectivity manipulation of two Benzylisoquinoline alkaloids O-methyltransferases from Stephania yunnanensis

Yuyang Zhang, Pan Yang, Chunsheng Tian, Yijie Zhai, Lukuan Hou, Junhai Xiao and Wenli Li

Bioorganic Chemistry, 2025, 157, 108238
DOI: 10.1016/j.bioorg.2025.108238

Computational metabolomics reveals overlooked chemodiversity of alkaloid scaffolds in Piper fimbriulatum

Tito Damiani, Joshua Smith, Téo Hebra, Milana Perković, Marijo Čičak, Alžběta Kadlecová, Vlastimil Rybka, Martin Dračínský and Tomáš Pluskal

The Plant Journal, 2025, 121
DOI: 10.1111/tpj.70086

Selection and functional identification of berberine biosynthetic genes in Berberis amurensis

Syed Basit Ali Shah, Lingzhe Kong, Yuzhuo Dong, Yaning Fu, Ya Tian, Yupeng Du, Zhichao Xu, Ge Bai and Zhoujie An

M, 2025, 4, 0
DOI: 10.48130/mpb-0025-0022

Functional Characterization of Two Methyltransferases Involved in Benzylisoquinoline Alkaloid Biosynthesis in Sinomenium acutum

Yuxin Qi, Xinlan Wang, Xiaotao Tuo, Yin Chen, Xina Yu, Yue Han, Lei Zhou, Jianing Deng, Shengxiong Huang, Xueshuang Huang and Peng Yang

Chemistry & Biodiversity, 2026, 23
DOI: 10.1002/cbdv.202503736

Virtual and CMC-Based Screening Identified Reticuline, an Intermediate of BIA Biosynthesis, as a Potential Agonist of D5R

Jing Mo, Zhihao Sun, Guoqing Xu, Guichun Zhang, Zhuangyuan Xie, Jinghao Zhao, Go Pei Heng, Zhaotong Cong, Liang Leng and Shilin Chen

Molecules, 2026, 31, 1285
DOI: 10.3390/molecules31081285

Advances in the biosynthesis of naturally occurring benzylisoquinoline alkaloids

Wanli Zhao, Jihua Liu and Yu Chen

Front. Plant Sci., 2025, 16
DOI: 10.3389/fpls.2025.1548471

Structural Elucidation and Engineering of the (S)‐scoulerine 2‐O‐Methyltransferase Enabling Regioselective Epiberberine Biosynthesis in Coptis chinensis

Jun Song, Di Liu, Shi Chen, Yufeng Tan, Siyu Wang, Shihan Li, Yutong Qin, Guofeng Li, Xufang Tian, Lan Yang, Chong Yuan, Junbo Gou, Wei Huang, Shilin Chen and Yifei Liu

Plant Biotechnology Journal, 2026
DOI: 10.1111/pbi.70650

Unveiling the multifaceted roles of crop secondary metabolites: From quality enhancement and stress resilience to molecular regulation and precision improvement

Hongwei Li, Hang Yan, Yingying Zhao, Qiqi Xiao, Nana Chen, Liwen Yang, Kuanping Deng and Delin Xu

Journal of Plant Physiology, 2026, 321, 154765
DOI: 10.1016/j.jplph.2026.154765

Deciphering the galantamine biosynthetic pathway in Lycoris species lays the foundation for plant chassis-based production

Lu Yuan, Qiong-Lin Chen, Qi Yang, Peng Zheng, Yan Liu, Rui Lin, Qing-Cheng Zou, Hang Fan, Xiao-Yuan Tao and Sheng-Chun Xu

Plant Physiology and Biochemistry, 2026, 233, 111252
DOI: 10.1016/j.plaphy.2026.111252

Gene duplication and clustering underlie the conservation and diversification of benzylisoquinoline alkaloid biosynthesis in plants

Changheng Shan, Xuan Zhou, Jiaojiao Zhu, Aatif Rashid, Jianhua Wang, Ning An, Changjian Zhang, Wenjuan Ji, Baosong Cai, Ke Wu, Sheng Wang and Zhenhua Liu

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-63175-x

Cycloastragenol in inflammation-related diseases: mechanisms, pharmacokinetics, and translational prospects

Chun Zhao, Xiuhua Yang, Man Yao, Xiaoxuan Song, Jingtong Dai and Pilong He

Front. Pharmacol., 2026, 16
DOI: 10.3389/fphar.2025.1732996

Complete Genome Sequence of Pithoascus kurdistanensis CBS 149789, an Endophytic Fungus Isolated from Papaver bracteatum

Sima Mohammadi, Jeff Gauthier, Guillaume Quang Henri Nguyen, Antony T. Vincent, Bahman Bahramnejad and Roger C. Levesque

JoF, 2025, 11, 861
DOI: 10.3390/jof11120861

Optimizing Plant Alkaloid Biosynthesis under Drought Stress: Regulatory Mechanisms and Biotechnological Strategies

Ziyi Guo, Sujing He, Xinying Zhong, Ning Yang and Delin Xu

Journal of Plant Physiology, 2025, 311, 154545
DOI: 10.1016/j.jplph.2025.154545

Functional characterization and protein engineering of a O-methyltransferase involved in benzylisoquinoline alkaloid biosynthesis of Stephania tetrandra

Zhoulu Wang, Kunlun Li, Jiaqian Mao, Hao Zhan, Yanting Wu, Zengyuan Wang, Yue Feng and Guoyin Kai

International Journal of Biological Macromolecules, 2025, 296, 139744
DOI: 10.1016/j.ijbiomac.2025.139744

Herbgenomics: Unraveling natural product biosynthesis in traditional Chinese medicine

Jing Wang, Jingyuan Song, Shilin Chen and Zhichao Xu

Chinese Herbal Medicines, 2026, 18, 3
DOI: 10.1016/j.chmed.2025.11.008

Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield

Lauren Narcross, Michael E. Pyne, Kaspar Kevvai, Ka-Hei Siu, John E. Dueber and Vincent J. J. Martin

ACS Synth. Biol., 2026, 15, 1187
DOI: 10.1021/acssynbio.5c00897

Evolution of key enzymes in the alkaloid biosynthetic pathway of Ranunculales

Zijian Dai, Feng Xu, Xiaomei Wei, Zhu Qiao, Weijia Wang, Zeyu Zhou, Guangning Zhang, Yang Dong, Xuzhen Li, Ying Hu and Dazhong Guo

ABD, 2026, 3, 31
DOI: 10.48130/abd-0026-0003

The key role of cytochrome P450s in the biosynthesis of plant derived natural products

Yawen Zhao, Zhenzhen Wu, Jiayao Li, Yaoxing Qi, Xiaoxiao Zhang and Chen Shen

Plant Physiology and Biochemistry, 2025, 222, 109695
DOI: 10.1016/j.plaphy.2025.109695

Regulatory mechanisms of secondary metabolite biosynthesis in medicinal plants under drought: advances and perspectives

Lingtiao Yao, Zhihan Wu, Zengyuan Wang, Yiqing Peng, Yang Yang, Yue Feng, Xiaolong Hao, Yongpeng Li and Guoyin Kai

Phytomedicine, 2026, 156, 158246
DOI: 10.1016/j.phymed.2026.158246

Unveiling the potential of natural TLR modulators in cancer immunotherapy

Wenying Zheng, Junming Chen, Jiajun Liu, Xintong Han, Siyuan Zhang, Chao Yang, Hua Yu, Wen Tan and Zhangfeng Zhong

Pharmacological Research, 2025, 221, 107969
DOI: 10.1016/j.phrs.2025.107969

Alkaloids in the <italic>Cephalotaxus</italic> plants and the biosynthesis of phenethylisoquinolines: a review

Hao Yan, Wei Li, Hao Wang, Shengzhuo Huang, Pengwei Chen, Jun Zeng, Jiahong Zhu, Yuji Li, Wenli Mei and Haofu Dai

Sci. Sin.-Chim, 2025, 55, 1220
DOI: 10.1360/SSC-2024-0273

Genome-wide identification and characterization of methyltransferase involved in benzylisoquinoline alkaloids biosynthesis from Menispermum dauricum

Yuwei Ma, Yaning Fu, Ge Bai, Shancen Zhao, Ya Tian, Ziyuan Zhao, Yan Wang, Zhichao Xu, Yong Chen, Zhoujie An and Ranran Gao

Chinese Herbal Medicines, 2026, 18, 97
DOI: 10.1016/j.chmed.2025.11.007

Metabolomic Insights into Sexual Multi-Morphism of Sinomenine Accumulation in Sinomenium acutum

Yanxian Luo, Wen Xu, Yanling Fan, Xinyu Ma, Qian Deng, Meng Li and Wei Sun

Plants, 2025, 14, 1885
DOI: 10.3390/plants14121885

A Modular Chemoenzymatic Cascade Simplifies Divergent Synthesis of Natural and Unnatural Benzylisoquinoline Alkaloids

Huiling Liu, Zhenbo Yuan, Yue Gao, Fei Li, Zhiwei Deng, Yan Zhang, Zhengshan Luo, Changmei Liu and Yijian Rao

Advanced Science, 2026, 13
DOI: 10.1002/advs.202513926

Multi-omics analysis reveals structural and transcriptional regulation specificity underlying differential benzylisoquinoline alkaloid accumulation in Coptis

Xufang Tian, Siyu Yang, Siyu Wang, Wei Li, Guofeng Li, Shi Zhang, Jin Wang, Di Liu and Yifei Liu

Horticulture Research, 2026, 13
DOI: 10.1093/hr/uhaf338