Cold argon plasmas for non-enzymatic digestion of macromolecules

Daniel McGill, Daniel Simon, Siva Ramadurai, Felicia Green and Zoltan Takats

Chem. Commun., 2026, 62, 2896
DOI: 10.1039/D5CC06333G

Ultrafast protein digestion using an immobilized enzyme reactor following high-resolution mass spectrometry analysis for rapid identification of abrin toxin

Meng Chen, Baoqiang Li, Wenlu Wei, Zhongyao Zhang, Lin Zhang, Cuiping Li and Qibin Huang

Analyst, 2024, 149, 3783
DOI: 10.1039/D4AN00406J

Quantitative neuropeptide analysis by mass spectrometry: advancing methodologies for biological discovery

Angel Erbey Ibarra, Wenxin Wu, Haoran Zhang and Lingjun Li

RSC Chem. Biol., 2025, 6, 1214
DOI: 10.1039/D5CB00082C

Recent developments in micro- and nanofluidic catalytic reactors utilizing ultra-small spaces

Ben-Yuan Zhang, Koki Yamamoto, Po-Yen Chen and Kyojiro Morikawa

Analyst, 2026, 151, 1597
DOI: 10.1039/D5AN01253H

Proteomic analysis of decellularized mice liver and kidney extracellular matrices

Anna-Maria Diedrich, Assal Daneshgar, Peter Tang, Oliver Klein, Annika Mohr, Olachi A. Onwuegbuchulam, Sabine von Rueden, Kerstin Menck, Annalen Bleckmann, Mazen A. Juratli, Felix Becker, Igor M. Sauer, Karl H. Hillebrandt, Andreas Pascher and Benjamin Struecker

J Biol Eng, 2024, 18
DOI: 10.1186/s13036-024-00413-8

Fit for Purpose Approach To Evaluate Detection of Amino Acid Substitutions in Shotgun Proteomics

Taylor J. Lundgren, Patricia L. Clark and Matthew M. Champion

J. Proteome Res., 2024, 23, 1263
DOI: 10.1021/acs.jproteome.3c00730

A New Detailed Mass Offset Search in MSFragger for Improved Interpretation of Complex PTMs

Carolina Rojas Ramírez, Fengchao Yu, Daniel A. Polasky and Alexey I. Nesvizhskii

J. Proteome Res., 2026, 25, 842
DOI: 10.1021/acs.jproteome.5c00775

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid‐2018–2022)

Sille Štěpánová and Václav Kašička

J of Separation Science, 2023, 46
DOI: 10.1002/jssc.202300043

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

Nerea Osinalde, Unai Alduntzin, Gorka Prieto and Ugo Mayor

FEBS Open Bio, 2026
DOI: 10.1002/2211-5463.70197

VOC-Based Probes, a New Set of Analytical Tools to Monitor Patient Health from Blood Sample. Proof of Concept on Tracking COVID-19 Infection

Estelle Blochouse, Rony Eid, Nahla Araji, Wei Tuo, Rémi Châtre, Sébastien Papot, Nicolas Lévêque, Raphaël Thuillier and Pauline Poinot

Anal. Chem., 2023, 95, 11572
DOI: 10.1021/acs.analchem.3c01732

Proteomics to Study Parchment Degradation – From Bulk to Spatial Analysis

Antonia Malissa, Federica Cappa, Manfred Schreiner and Martina Marchetti-Deschmann

Restaurator. International Journal for the Preservation of Library and Archival Material, 2024, 45, 141
DOI: 10.1515/res-2024-0003

How Can Proteomics Help to Elucidate the Pathophysiological Crosstalk in Muscular Dystrophy and Associated Multi-System Dysfunction?

Paul Dowling, Capucine Trollet, Elisa Negroni, Dieter Swandulla and Kay Ohlendieck

Proteomes, 2024, 12, 4
DOI: 10.3390/proteomes12010004

Recent Developments and Applications of Capillary and Microchip Electrophoresis in Proteomics and Peptidomics (2023–2025)

Sille Štěpánová and Václav Kašička

J of Separation Science, 2026, 49
DOI: 10.1002/jssc.70395

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Václav Kašička

Electrophoresis, 2024, 45, 165
DOI: 10.1002/elps.202300152

Relative quantification of proteins and post-translational modifications in proteomic experiments with shared peptides: a weight-based approach

Mateusz Staniak, Ting Huang, Amanda M Figueroa-Navedo, Devon Kohler, Meena Choi, Trent Hinkle, Tracy Kleinheinz, Robert Blake, Christopher M Rose, Yingrong Xu, Pierre M Jean Beltran, Liang Xue, Małgorzata Bogdan, Olga Vitek and Jianlin Cheng

Bioinformatics, 2025, 41
DOI: 10.1093/bioinformatics/btaf046

Ion Activation Methods for Top‐Down Proteomics

Jada N. Walker and Jennifer S. Brodbelt

Mass Spectrometry Reviews, 2026
DOI: 10.1002/mas.70023

The role of multi-omics in biomarker discovery, diagnosis, prognosis, and therapeutic monitoring of tissue repair and regeneration processes

Jiamei Liu, Liyu Yang, Dongze Liu, Qianlong Wu, Yuanqi Yu, Xiaoming Huang, Jianjun Li and Shengye Liu

Journal of Orthopaedic Translation, 2025, 54, 131
DOI: 10.1016/j.jot.2025.07.006

Distinct Proteomic and Glycosylation Signatures Differentiate A549 Tumor and BEAS-2B Nontumor Cell Line–Derived Small Extracellular Vesicles

Mirjam Balbisi, Tamás Langó, Virág Nikolett Horváth, Domonkos Pál, Gitta Schlosser, Gábor Kecskeméti, Zoltán Szabó, Kinga Ilyés, Nikolett Nagy, Otília Tóth, Jing Zheng, Guinevere S.M. Lageveen-Kammeijer, Tamás Visnovitz, Zoltán Varga, Beáta G. Vértessy and Lilla Turiák

Molecular & Cellular Proteomics, 2026, 25, 101524
DOI: 10.1016/j.mcpro.2026.101524

Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology

Paul Dowling, Dieter Swandulla and Kay Ohlendieck

Cells, 2023, 12, 2560
DOI: 10.3390/cells12212560

‘Omics technologies for the elucidation of the molecular mechanisms related to carbapenem-resistant and extended spectrum β-lactamase-producing Klebsiella pneumoniae

Breyer Woodland, Piklu Roy Chowdhury, Matthew B. O’Rourke and Matthew P. Padula

Talanta, 2026, 296, 128480
DOI: 10.1016/j.talanta.2025.128480

Predicting and Treating Pulmonary Fibrosis with Proteomic Biomarker Investigations

Giulia Raineri, Anna Valeria Samarelli, Roberto Tonelli, Valentina Masciale, Beatrice Aramini, Tiziana Petrachi, Giulia Bruzzi, Filippo Gozzi, Ester Trasforini, Angela Esposito, Filippo Azzali, Massimo Dominici, Albino Eccher, Stefania Cerri and Enrico Clini

Biomedicines, 2025, 13, 2656
DOI: 10.3390/biomedicines13112656

Mass Spectrometry Strategies for O-Glycoproteomics

Amanda Helms and Jennifer S. Brodbelt

Cells, 2024, 13, 394
DOI: 10.3390/cells13050394

The Enzyme Effect: Broadening the Horizon of MS Optimization to Nontryptic Digestion in Proteomics

Kinga Nagy, Péter Sándor, Károly Vékey, László Drahos and Ágnes Révész

J. Am. Soc. Mass Spectrom., 2025, 36, 299
DOI: 10.1021/jasms.4c00396

Diagnostics of Autoimmune Hepatitis Enabled by Non‐Invasive Clinical Proteomics

Anne‐Sofie Houlberg Jensen, Henriette Ytting, Annelaura Bach Nielsen, Mikkel Parsberg Werge, Elias Badal Rashu, Liv Eline Hetland, Mira Thing, Puria Nabilou, Johan Burisch, Anders Ellekær Junker, Lise Hobolth, Christian Mortensen, Flemming Tofteng, Flemming Bendtsen, Søren Møller, Mogens Vyberg, Reza Rafiolsadat Serizawa, Marie Winther‐Sørensen, Jesper Sloth Kellemann, Lise Lotte Gluud and Nicolai Jacob Wewer Albrechtsen

Aliment Pharmacol Ther, 2025, 62, 901
DOI: 10.1111/apt.70273

Artefact Profiling: Panomics Approaches for Understanding the Materiality of Written Artefacts

Marina Creydt and Markus Fischer

Molecules, 2023, 28, 4872
DOI: 10.3390/molecules28124872

Modanovo: A Unified Model for Post-translational Modification-Aware De Novo Sequencing Using Experimental Spectra From In Vivo and Synthetic Peptides

Daniela Klaproth-Andrade, Yanik Bruns, Wassim Gabriel, Christian Nix, Valter Bergant, Andreas Pichlmair, Mathias Wilhelm and Julien Gagneur

Molecular & Cellular Proteomics, 2026, 25, 101501
DOI: 10.1016/j.mcpro.2025.101501

Insights on Wet and Dry Workflows for Human Gut Metaproteomics

Valeria Marzano, Stefano Levi Mortera and Lorenza Putignani

Proteomics, 2025, 25, 230
DOI: 10.1002/pmic.202400242

Data mining antibody sequences for database searching in bottom-up proteomics

Xuan-Tung Trinh, Rebecca Freitag, Konrad Krawczyk and Veit Schwämmle

ImmunoInformatics, 2024, 15, 100042
DOI: 10.1016/j.immuno.2024.100042

Identification of gravity-responsive proteins in the femur of spaceflight mice using a quantitative proteomic approach

Kenji Egashira, Yoko Ino, Yusuke Nakai, Takashi Ohira, Tomoko Akiyama, Kayano Moriyama, Yu Yamamoto, Mitsuo Kimura, Akihide Ryo, Tomoyuki Saito, Yutaka Inaba, Hisashi Hirano, Ken Kumagai and Yayoi Kimura

Journal of Proteomics, 2023, 288, 104976
DOI: 10.1016/j.jprot.2023.104976

Top‐Down Proteomics: Why and When?

Philipp T. Kaulich and Andreas Tholey

Proteomics, 2025, 25, 6
DOI: 10.1002/pmic.202400338

Greenness of proteomic sample preparation and analysis techniques for biopharmaceuticals

Katarina Marakova, Radovan Tomasovsky, Martina Opetova and Kevin A. Schug

TrAC Trends in Analytical Chemistry, 2024, 171, 117490
DOI: 10.1016/j.trac.2023.117490

Computational and Systems Biology Advances to Enable Bioagent Agnostic Signatures

Andy Lin, Cameron M. Torres, Errett C. Hobbs, Jaydeep Bardhan, Stephen B. Aley, Charles T. Spencer, Karen L. Taylor and Tony Chiang

Health Security, 2024, 22, 130
DOI: 10.1089/hs.2023.0076

Relevance of proteomics and metabolomics approaches to overview the tumorigenesis and better management of cancer

Pooja Singh, Yashika W. Dhir, Shagun Gupta, Ankur Kaushal, Deepak Kala, Rupak Nagraiik, Naveen K. Kaushik, Md Salik Noorani, Abdul R. Asif, Bharat Singh, Shahbaz Aman and Sunny Dhir

3 Biotech, 2025, 15
DOI: 10.1007/s13205-025-04222-8

High-resolution mass spectrometry measurement of N-terminal carbamylated hemoglobin as a potential marker for chronic diseases with elevated blood urea levels

Fangjun Chen, Priscilla S.-W. Yeung, Carolyn V. Wong and Ruben Y. Luo

Journal of Mass Spectrometry and Advances in the Clinical Lab, 2025, 35, 8
DOI: 10.1016/j.jmsacl.2025.01.001

Top-Down Proteomics and the Challenges of True Proteoform Characterization

Allen Po and Claire E. Eyers

J. Proteome Res., 2023, 22, 3663
DOI: 10.1021/acs.jproteome.3c00416

Efficient and rapid digestion of proteins with a dual-enzyme microreactor featuring 3-D pores formed by dopamine/polyethyleneimine/acrylamide-coated KIT-6 molecular sieve

Fang-Fang Yuan, Pei Wang, Xiao-Jie Han, Ting-Ting Qin, Xin Lu and Hai-Jiao Bai

Sci Rep, 2024, 14
DOI: 10.1038/s41598-024-65045-w

Negative-Ion Electron Capture Dissociation of MALDI-Generated Peptide Anions

Steven A. DeFiglia, Carson W. Szot and Kristina Håkansson

Anal. Chem., 2024, 96, 8800
DOI: 10.1021/acs.analchem.4c01292

Induced-volatolomics, a new research field in chemical biology

Estelle Blochouse, Rony Eid, Elsa Cannoni, Rémi Châtre, Claude Geffroy, Isabelle Opalinski, Sébastien Papot and Pauline Poinot

Comptes Rendus. Chimie, 2025, 26, 93
DOI: 10.5802/crchim.252

Ultrafast Proteomics

Ivan I. Fedorov, Sergey A. Protasov, Irina A. Tarasova and Mikhail V. Gorshkov

Biochemistry Moscow, 2024, 89, 1349
DOI: 10.1134/S0006297924080017

Omics approaches to unravel insecticide resistance mechanism in Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

Muhammad Aqil Fitri Rosli, Sharifah Nabihah Syed Jaafar, Kamalrul Azlan Azizan, Salmah Yaakop and Wan Mohd Aizat

PeerJ, 2024, 12, e17843
DOI: 10.7717/peerj.17843

Estimating the time of human decomposition based on skeletal muscle biopsy samples utilizing an untargeted LC–MS/MS-based proteomics approach

Lana Brockbals, Samara Garrett-Rickman, Shanlin Fu, Maiken Ueland, Dennis McNevin and Matthew P. Padula

Anal Bioanal Chem, 2023, 415, 5487
DOI: 10.1007/s00216-023-04822-4

3D printed hardware for automation of proteomics sample preparation at the Meso-Scale

Sadie R. Schultz and Matthew M. Champion

Talanta Open, 2025, 12, 100505
DOI: 10.1016/j.talo.2025.100505

Acceleration, simplification and potential parallelization of digital polymers sequencing by coupling tandem mass spectrometry with ion mobility

Isaure Sergent, Georgette Obeid, Thibault Schutz, Jean-François Lutz and Laurence Charles

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-66762-0

Mass spectrometry–based proteomic approaches for postmortem interval estimation: A systematic review

Rakshita Gautam, Akansha Das, Sachil Kumar and Wahid Ali

Journal of Forensic and Legal Medicine, 2026, 118, 103096
DOI: 10.1016/j.jflm.2026.103096

Toward single-molecule protein sequencing using nanopores

Chunzhe Lu, Andrea Bonini, Jakob H. Viel and Giovanni Maglia

Nat Biotechnol, 2025, 43, 312
DOI: 10.1038/s41587-025-02587-y

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Xinyan Fu, Jie Hong, Yanbing Zhai, Kefu Liu and Wei Xu

Anal. Chem., 2023, 95, 10137
DOI: 10.1021/acs.analchem.3c00532

High-Throughput Workflow for Detergent-free Cell-Based Proteomic Characterization

Saeed Seyedmohammad, Alejandro Rivas, Maxim Zhgamadze, Ali Haghani, Simion Kreimer, Ajay Bharadwaj, Niveda Sundararaman, Sameer Vasantgadkar, Kasturi Pal, Eugenio Daviso, Aleksandr Stotland, Chris Murray, Koen Raedschelders, Sudha Savant and Jennifer E. Van Eyk

J. Proteome Res., 2025, 24, 3762
DOI: 10.1021/acs.jproteome.4c00892

Proteomics and metabolomics for assessing metabolite changes, safety, and adulteration in milk products

Chaeeun Moon, Won Young Oh and JuDong Yeo

Food Sci Biotechnol, 2025
DOI: 10.1007/s10068-025-02030-7

Top-Down Proteomic Profiling of Protein Corona by High-Throughput Capillary Isoelectric Focusing-Mass Spectrometry

Reyhane Tabatabaeian Nimavard, Seyed Amirhossein Sadeghi, Morteza Mahmoudi, Guijie Zhu and Liangliang Sun

J. Am. Soc. Mass Spectrom., 2025, 36, 778
DOI: 10.1021/jasms.4c00463

Progress and prospects of biomolecule mass spectrometry based on advanced ultraviolet light source

Fangjun Wang, Zheyi Liu, Zhibin Yin, Pan Luo, Shirui Yang, Chunlei Xiao and Xueming Yang

Sci. Sin.-Chim, 2023, 53, 2290
DOI: 10.1360/SSC-2023-0143

Development of an extraction method for the identification of peptides in the spermosphere of common bean (Phaseolus vulgaris L.)

Chandrodhay Saccaram, Benoit Bernay, Céline Brosse, Boris Collet, Shuang Peng, Delphine Sourdeval, Tracy François, Massimiliano Corso and Loïc Rajjou

Seed Sci. Res., 2025, 35, 49
DOI: 10.1017/S0960258525000030

Innovations in heart failure management: The role of cutting-edge biomarkers and multi-omics integration

Jose Mesquita Bastos, Beatriz Colaço, Rui Baptista, Cristina Gavina and Rui Vitorino

Journal of Molecular and Cellular Cardiology Plus, 2025, 11, 100290
DOI: 10.1016/j.jmccpl.2025.100290

Mass Spectrometry-Based Proteomics for Seafood Allergen Detection and Quantification: Current Trends and Technological Frontiers

Manuel G. Amado, Manuel Pazos and Mónica Carrera

IJMS, 2025, 26, 8962
DOI: 10.3390/ijms26188962

TCEP‐Mediated Protein Hydrolysis: Highly Selective Cleavage C‐Terminal to Aspartic Acid

Daniel S. McCracken, David J. Foreman, Kyle G. Esposito, Alyssa Q. Stiving, Benjamin W. Roose, Danielle V. Miller and Feras Hatahet

Rapid Comm Mass Spectrometry, 2026, 40
DOI: 10.1002/rcm.70051

Bacterial Proteomics and Antibiotic Resistance Identification: Is Single-Cell Analysis a Worthwhile Pursuit?

Navid J. Ayon

Pathogens, 2025, 14, 1127
DOI: 10.3390/pathogens14111127

Using Proteins As Markers for Anabolic Steroid Abuse: A New Perspective in Doping Control?

Maxime Sansoucy and Jean-François Naud

Chem. Res. Toxicol., 2023, 36, 1168
DOI: 10.1021/acs.chemrestox.3c00166

Mass Spectrometry for Lysine Methylation: Principles, Progress, and Prospects

Mackenzie G. Cumming and Kyle K. Biggar

Biomedicines, 2025, 13, 2825
DOI: 10.3390/biomedicines13112825

Post-Translationally Modified Proteoforms as Biomarkers: From Discovery to Clinical Use

Ruben Y Luo, Priscilla S W Yeung, Morgan W Mann, Lichao Zhang, Yifei K Yang and Andrew N Hoofnagle

Clinical Chemistry, 2025, 71, 1111
DOI: 10.1093/clinchem/hvaf094

Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels

Maor Arad, Connor Frey, Ronald Balagtas, Rhien Hare, Kenneth Ku, Dario Jereb, Zach Nestman, Anoop Sidhu, Yuming Shi, Osei Fordwour, Kyung-Mee Moon, Leonard J. Foster and Golfam Ghafourifar

Anal. Chem., 2024, 96, 18880
DOI: 10.1021/acs.analchem.4c04846

Unveiling Tumorigenesis Mechanisms and Drug Therapy in Neuroblastoma by Mass Spectrometry Based Proteomics

Keyi Ren, Yu Wang, Minmin Zhang, Ting Tao and Zeyu Sun

Children, 2024, 11, 1323
DOI: 10.3390/children11111323

Application of Proteomics in the Study of Toxicology and Toxic Markers of Traditional Chinese Medicines

Yang Yadi, Shang Ziyi, Hu Bin, Sun Ye, Li Jingjing, Ma Yujin, Jiang Hongwei, Liu Chuanxin and Li Xuejiao

Biomedical Chromatography, 2025, 39
DOI: 10.1002/bmc.70216

Streamlined integrated protein isoelectric focusing using microfluidic paper-based device

Geovana M. Mendes, Fanny d'Orlye, Laura Trapiella-Alfonso, Gabriela R.M. Duarte and Anne Varenne

Journal of Chromatography A, 2024, 1732, 465222
DOI: 10.1016/j.chroma.2024.465222

Recent implementations of data-independent acquisition for cancer biomarker discovery in biological fluids

Annie Ha, Michael Woolman, Matthew Waas, Meinusha Govindarajan and Thomas Kislinger

Expert Review of Proteomics, 2025, 22, 163
DOI: 10.1080/14789450.2025.2491355

Bottom-Up Proteomics: Advancements in Sample Preparation

Van-An Duong and Hookeun Lee

IJMS, 2023, 24, 5350
DOI: 10.3390/ijms24065350

Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix

Paul Dowling, Stephen Gargan, Margit Zweyer, Michael Henry, Paula Meleady, Dieter Swandulla and Kay Ohlendieck

Eur J Transl Myol, 2024
DOI: 10.4081/ejtm.2024.12564