Issue 39, 2024

Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

Abstract

Through the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events.

Graphical abstract: Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

Supplementary files

Article information

Article type
Communication
Submitted
08 3 2024
Accepted
17 4 2024
First published
18 4 2024

Chem. Commun., 2024,60, 5197-5200

Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA

C. Lin, W. Chen, L. Liu and L. Cheng, Chem. Commun., 2024, 60, 5197 DOI: 10.1039/D4CC01106F

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