Issue 4, 2022
From the journal:

Analytical Methods

Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability†

Gianmarco D. Suarez,af   Domenick A. Suarez,f   Yuki Yu Kiu Tang,f   Jing-Xuan Zhang,d   Jun Li, ORCID logo de   Stefan Nagl ORCID logo a  and  Peter Pak-Hang Cheung ORCID logo *bcf  

* Corresponding authors

a Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong

b Department of Chemical Pathology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong
E-mail: ppcheung@cuhk.edu.hk

c Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Li Ka Shing Medical Sciences Building, Shatin, New Territories, Hong Kong

d Department of Chemistry, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China

e Department of Chemistry and Key Laboratory of Organic Optoelectronics & Molecular Engineering of Ministry of Education, Tsinghua University, Beijing 100084, China

f Quommni Technologies Limited, Hong Kong

Abstract

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.

Graphical abstract: Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D1AY01395E
Article type
Communication
Submitted
15 8 2021
Accepted
17 12 2021
First published
20 12 2021

Anal. Methods, 2022,14, 378-382

Permissions

Request permissions

Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

G. D. Suarez, D. A. Suarez, Y. Y. Kiu Tang, J. Zhang, J. Li, S. Nagl and P. P. Cheung, Anal. Methods, 2022, 14, 378 DOI: 10.1039/D1AY01395E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements