Secondary structure changes as the potential H2 sensing mechanism of group D [FeFe]-hydrogenases

Ivan Voloshyn; Conrad Schumann; Princess R. Cabotaje; Afridi Zamader; Henrik Land; Moritz Senger

doi:10.1039/D4CC03098B

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Secondary structure changes as the potential H₂ sensing mechanism of group D [FeFe]-hydrogenases†

Ivan Voloshyn,

^a Conrad Schumann,^b Princess R. Cabotaje,^b Afridi Zamader,

‡^b Henrik Land

^b and Moritz Senger

*^ab

Author affiliations

* Corresponding authors

^a Department of Chemistry – BMC, Biochemistry, Uppsala University, 75120 Uppsala, Sweden
E-mail: moritz.senger@kemi.uu.se

^b Department of Chemistry – Ångström Laboratory, Molecular Biomimetics, Uppsala University, 75120 Uppsala, Sweden

Abstract

[FeFe]-hydrogenases function as both H₂ catalysts and sensors. While catalysis is well investigated, details regarding the H₂ sensing mechanism are limited. Here, we relate protein structure changes to H₂ sensing, similar to light-driven bio-sensors. Our results highlight how identical cofactors incorporated in alternative protein scaffolds serve different functions in nature.

This article is part of the themed collection: ChemComm Milestones – First Independent Articles

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Article information

DOI: https://doi.org/10.1039/D4CC03098B
Article type: Communication
Submitted: 25 6 2024
Accepted: 04 9 2024
First published: 05 9 2024
This article is Open Access

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Chem. Commun., 2024,60, 10914-10917

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Secondary structure changes as the potential H₂ sensing mechanism of group D [FeFe]-hydrogenases

I. Voloshyn, C. Schumann, P. R. Cabotaje, A. Zamader, H. Land and M. Senger, Chem. Commun., 2024, 60, 10914 DOI: 10.1039/D4CC03098B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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