Issue 64, 2024

A lanthanide tag for a complementary set of pseudocontact shifts

Abstract

Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.

Graphical abstract: A lanthanide tag for a complementary set of pseudocontact shifts

Supplementary files

Article information

Article type
Communication
Submitted
20 6 2024
Accepted
16 7 2024
First published
16 7 2024
This article is Open Access
Creative Commons BY license

Chem. Commun., 2024,60, 8458-8461

A lanthanide tag for a complementary set of pseudocontact shifts

L. Topping, A. P. Welegedara, M. Judd, E. H. Abdelkader, N. Cox, G. Otting and S. J. Butler, Chem. Commun., 2024, 60, 8458 DOI: 10.1039/D4CC03007A

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