Issue 25, 2018

Genetically encoded tags for real time dissection of protein assembly in living cells

Abstract

Simple methods with straightforward readouts that enable real-time interrogation of protein quaternary structure are much needed to facilitate the physicochemical characterization of proteins at the single-cell level. After screening over a series of microtubule (MT) binders, we report herein the development of two genetically encoded tags (designated as “MoTags” for the monomer/oligomer detection tag) that can be conveniently fused to a given protein to probe its oligomeric state in cellulo when combined with routine fluorescence microscopy. In their monomeric form, MoTags are evenly distributed in the cytosol; whereas oligomerization enables MoTags to label MT or track MT tips in an oligomeric state-dependent manner. We demonstrate here the broad utility of engineered MoTags to aid the determination of protein oligomeric states, dissection of protein structure and function, and monitoring of protein–target interactions under physiological conditions in living cells.

Graphical abstract: Genetically encoded tags for real time dissection of protein assembly in living cells

Supplementary files

Article information

Article type
Edge Article
Submitted
21 2 2018
Accepted
22 5 2018
First published
23 5 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 5551-5555

Genetically encoded tags for real time dissection of protein assembly in living cells

G. Ma, Q. Zhang, L. He, N. T. Nguyen, S. Liu, Z. Gong, Y. Huang and Y. Zhou, Chem. Sci., 2018, 9, 5551 DOI: 10.1039/C8SC00839F

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