Issue 85, 2023

Development of a whole-cell biosensor for β-lactamase inhibitor discovery

Abstract

The production of β-lactamases by bacterial pathogens endangers antimicrobial therapy, and new inhibitors for β-lactamases are urgently needed. We report the development of a luminescent-based biosensor that quantifies β-lactamase inhibition in a cellular context, based on the activation of transcriptional factor AmpR following the exposure of bacterial cells to β-lactams. This rapid method can account for factors like membrane permeability and can be employed to identify new β-lactamase inhibitors.

Graphical abstract: Development of a whole-cell biosensor for β-lactamase inhibitor discovery

Supplementary files

Article information

Article type
Communication
Submitted
25 7 2023
Accepted
02 10 2023
First published
02 10 2023

Chem. Commun., 2023,59, 12707-12710

Development of a whole-cell biosensor for β-lactamase inhibitor discovery

M. A. Jeffs, R. A. V. Gray, P. M. Sheth and C. T. Lohans, Chem. Commun., 2023, 59, 12707 DOI: 10.1039/D3CC03583B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements