Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase

Anne Volbeda; Patricia Amara; Marina Iannello; Antonio L. De Lacey; Christine Cavazza; Juan Carlos Fontecilla-Camps

doi:10.1039/C3CC43619E

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Structural foundations for the O₂ resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase†

Anne Volbeda,*^a Patricia Amara,^a Marina Iannello,^a Antonio L. De Lacey,^b Christine Cavazza^a and Juan Carlos Fontecilla-Camps^a

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* Corresponding authors

^a Metalloproteins Unit, Institut de Biologie Structurale J.P. Ebel, CEA, CNRS, Université Grenoble Alpes UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble, France
E-mail: anne.volbeda@ibs.fr
Fax: +33 4 3878 5122
Tel: +33 4 3878 9606

^b Instituto de Catálisis y Petroleoquímica, CSIC, c/Marie Curie 2, 28049 Madrid, Spain

Abstract

This study shows how the NiFeSe site of an anaerobically purified O₂-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.

This article is part of the themed collection: Electrocatalytic hydrogen evolution

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Article information

DOI: https://doi.org/10.1039/C3CC43619E
Article type: Communication
Submitted: 14 5 2013
Accepted: 13 6 2013
First published: 13 6 2013

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Chem. Commun., 2013,49, 7061-7063

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Structural foundations for the O₂ resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase

A. Volbeda, P. Amara, M. Iannello, A. L. De Lacey, C. Cavazza and J. C. Fontecilla-Camps, Chem. Commun., 2013, 49, 7061 DOI: 10.1039/C3CC43619E

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