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Abstract | Cited by

Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein–oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.

Graphical abstract: The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

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