Issue 86, 2021

Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols

Abstract

We report a chemiluminescent probe (CLPT1) that permits the paired detection of tyrosinase (Tyr) and biological thiols. Tyr only leads to a poor chemiluminescence response, a finding ascribed to the formation of a stable o-benzoquinone intermediate. The addition of glutathione (GSH), or ascorbate to the o-benzoquinone intermediate results in thiol conjugation or reduction to this intermediate, respectively. This produces a strong chemiluminescence response. Thiol co-dependence was demonstrated in live cells using the cell permeable analogue, CLPT3. The present chemiluminescence-based strategy allows the concurrent detection of tyrosinase activity and biological thiols.

Graphical abstract: Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols

Supplementary files

Article information

Article type
Communication
Submitted
15 ៩ 2021
Accepted
04 ១០ 2021
First published
04 ១០ 2021

Chem. Commun., 2021,57, 11386-11389

Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols

O. Shelef, A. C. Sedgwick, S. Pozzi, O. Green, R. Satchi-Fainaro, D. Shabat and J. L. Sessler, Chem. Commun., 2021, 57, 11386 DOI: 10.1039/D1CC05217A

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