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An artificial protein-probe hybrid, Cm–Np-B@BSA, was prepared via host–guest interactions between hydrogen peroxide (H2O2)-responsive Cm–Np-B molecule and bovine serum albumin (BSA). The Cm–Np-B@BSA probe exhibited high sensitivity and selectivity towards H2O2 under physiological conditions and had excellent biocompatibility, allowing for sensitive ratiometric detection and imaging of endogenous H2O2 in live cells.

Graphical abstract: An artificial protein-probe hybrid as a responsive probe for ratiometric detection and imaging of hydrogen peroxide in cells

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