Christof Domnick, Gregor Hagelueken, Frank Eggert, Olav Schiemann and Stephanie Kath-Schorr
Org. Biomol. Chem., 2019,17, 1805-1808
DOI:
10.1039/C8OB02597E,
Communication
The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.