Issue 49, 2025
From the journal:

Chemical Communications

Discovery and evaluation of a novel selective fluorescent substrate for CYP3A4 based on sulfoxidation characteristics†

Chao-Hua Yan,‡a   Gui-Lin Wei,‡ac   Zhuo-An Jin‡,a   Tian-Chi Lu,a   Xiao-dong Li,a   Ji-Cong Wu,a   Chun-Yang Yu, ORCID logo d   Li-Wei Zou ORCID logo *a  and  Ling Yang*ab  

* Corresponding authors

a Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China
E-mail: chemzlw@163.com, yling@shutcm.edu.cn

b Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, China

c School of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, Panjin 124221, China

d School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai, China

Abstract

This study for the first time reports the design and application of a novel type of selective fluorescent substrate NESF for cytochrome P450 3A4 (CYP3A4) based on sulfoxidation reaction. NESF has been successfully applied for imaging endogenous CYP3A4 in living cells and for high-throughput screening of CYP3A4 inhibitors.

Graphical abstract: Discovery and evaluation of a novel selective fluorescent substrate for CYP3A4 based on sulfoxidation characteristics

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Article information

DOI
https://doi.org/10.1039/D5CC01981H
Article type
Communication
Submitted
09 ៤ 2025
Accepted
14 ៥ 2025
First published
15 ៥ 2025

Chem. Commun., 2025,61, 8871-8874

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Discovery and evaluation of a novel selective fluorescent substrate for CYP3A4 based on sulfoxidation characteristics

C. Yan, G. Wei, Z. Jin‡, T. Lu, X. Li, J. Wu, C. Yu, L. Zou and L. Yang, Chem. Commun., 2025, 61, 8871 DOI: 10.1039/D5CC01981H

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