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A fluorescence sensing system for inorganic pyrophosphate based on ligand exchange of the Ce(III) complex of a xanthene-type probe is developed. This sensing system is successfully applied to the fluorescence detection of polymerase-catalyzed DNA amplification using loop-mediated isothermal amplification.

Graphical abstract: Coordination ligand exchange of a xanthene probe–Ce(iii) complex for selective fluorescence sensing of inorganic pyrophosphate

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