Issue 6, 2019

Label free electrochemical detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterials

Abstract

This paper reports label free sub-femtomolar detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterial based electrochemical biosensors. Herein, we have demonstrated two separate synthesis schemes for the perovskite material, one being a hydrothermal synthesis method, and the other being electrochemical deposition. In this work, for the electrochemical detection of troponin T with the hydrothermally synthesized ZnSnO3, we have used a glassy carbon electrode (GCE), on to which the nanomaterials are dropcasted so as to create a heterogeneous working electrode. In a separate scheme, we have used indium tin oxide coated polyethylene terephthalate (ITO/PET) substrates as the working electrodes, on to which a thin film of ZnSnO3 nanomaterial has been deposited electrochemically. Subsequently, the capture antibodies corresponding to the targeted cardiac troponin T are immobilized on to the surface functionalized working electrodes using NHS/EDC chemistry. The electrochemical detection of the target analyte has been performed in the concentration range of 1 fg mL−1 to 1 μg mL−1, using electrochemical impedance spectroscopy. For the GCE and ITO/PET based bioelectrodes, the approximated limits of detection are found to be 0.187 fg mL−1 and 0.571 fg mL−1 respectively, whereas the respective values of sensitivity of the same are 35.25 kΩ (μg mL−1)−1 cm−2 and 8.813 kΩ (μg mL−1)−1 cm−2.

Graphical abstract: Label free electrochemical detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterials

Supplementary files

Article information

Article type
Paper
Submitted
30 Қар. 2018
Accepted
04 Қаң. 2019
First published
04 Қаң. 2019

Anal. Methods, 2019,11, 744-751

Label free electrochemical detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterials

P. Supraja, V. Sudarshan, S. Tripathy, A. Agrawal and S. G. Singh, Anal. Methods, 2019, 11, 744 DOI: 10.1039/C8AY02617C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements