Issue 14, 2016

Dual emissive analogue of deoxyuridine as a sensitive hydration-reporting probe for discriminating mismatched from matched DNA and DNA/DNA from DNA/RNA duplexes

Abstract

Fluorescent nucleosides with high sensitivity to H-bonding are of great interest not only to investigate DNA hydration and DNA–protein interactions and dynamics, but also to develop screening assays. Here, we report on the incorporation of a dual emissive uridine analogue based on the 3-hydroxychromone scaffold (3HC) into DNA oligonucleotides of different sequences and the characterization of the spectroscopic properties of the labelled oligonucleotides in matched and mismatched duplexes and in duplexes of different forms. The probe exhibits two-colour emission and spectral λ-shift, which vary as a function of the local hydration of the 3HC. It provides the highest sensitivity together with calibration on the molecular level, which allows discriminating between matched and mismatched dsDNA, as well as between B- and A-forms of DNA/DNA and DNA/RNA duplexes. We suggest that the physical basis of such behavior is the change of hydration in the close proximity of the fluorescent base emitter which appears ideally suitable for such sensing.

Graphical abstract: Dual emissive analogue of deoxyuridine as a sensitive hydration-reporting probe for discriminating mismatched from matched DNA and DNA/DNA from DNA/RNA duplexes

Supplementary files

Article information

Article type
Paper
Submitted
20 Қаз. 2015
Accepted
21 Жел. 2015
First published
22 Жел. 2015

J. Mater. Chem. C, 2016,4, 3010-3017

Dual emissive analogue of deoxyuridine as a sensitive hydration-reporting probe for discriminating mismatched from matched DNA and DNA/DNA from DNA/RNA duplexes

N. P. F. Barthes, K. Gavvala, D. Dziuba, D. Bonhomme, J. Karpenko, A. S. Dabert-Gay, D. Debayle, A. P. Demchenko, R. Benhida, B. Y. Michel, Y. Mély and A. Burger, J. Mater. Chem. C, 2016, 4, 3010 DOI: 10.1039/C5TC03427B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements