Issue 23, 2016

Hairpin ribozyme mediated RNA recombination

Abstract

We have engineered a hairpin ribozyme that in a two-step reaction supports RNA recombination by catalysing the cleavage of two non-functional RNA precursors (first step) followed by the recombination of two of the cleavage fragments into a functional RNA (second step). The recombination product (yield: 76%) is a fully functional hammerhead ribozyme.

Graphical abstract: Hairpin ribozyme mediated RNA recombination

  • This article is part of the themed collection: Foldamers

Supplementary files

Article information

Article type
Communication
Submitted
14 Қаң. 2016
Accepted
16 Ақп. 2016
First published
16 Ақп. 2016

Chem. Commun., 2016,52, 4365-4368

Hairpin ribozyme mediated RNA recombination

R. Hieronymus, S. P. Godehard, D. Balke and S. Müller, Chem. Commun., 2016, 52, 4365 DOI: 10.1039/C6CC00383D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements