Issue 18, 2023

Dynamic display of cell targeting motifs via natural glycopeptide recognition for cancer cell isolation

Abstract

Reversible binding of cell targeting motifs on biomaterial surfaces can be used to mimic the dynamic bioactivities of the extracellular matrix (ECM), thus showing potential to modulate cell behaviours in physiopathologic processes. As the markers of tumour metastasis, circulating tumour cells (CTCs) are especially essential for the diagnosis of cancer. Dynamic display of cancer targeting motifs on biometric interfaces thus enables specific isolation of rare cancer cells and delivery of critical information for cancer therapy. In this work, we report a dynamic biointerface for the reversible binding of cancer-targeting motifs on quartz through molecular recognition between the natural glycopeptide vancomycin (Van) and the D-alanine-D-alanine (AA) dipeptide. This active surface could not only realize specific cancer cell adhesion but also induce nondestructive cell detachment by the competitive addition of the free AA dipeptide. The cell isolation efficiency in the medium could reach 70% within 120 min, showing high specificity and separation efficiency. Furthermore, the dynamic display of cancer-targeting motifs on magnetic beads enabled magnetic cancer cell capture and AA dipeptide-triggered cell release. Therefore, the natural dynamic strategy using noninvasive glycopeptide recognition holds promise for rare cell detection, isolation and cell-based disease diagnosis.

Graphical abstract: Dynamic display of cell targeting motifs via natural glycopeptide recognition for cancer cell isolation

Supplementary files

Article information

Article type
Research Article
Submitted
06 Мау. 2023
Accepted
15 Мау. 2023
First published
17 Мау. 2023

Mater. Chem. Front., 2023,7, 4050-4062

Dynamic display of cell targeting motifs via natural glycopeptide recognition for cancer cell isolation

W. He, Z. Yao, Y. Diao, M. Wang and G. Pan, Mater. Chem. Front., 2023, 7, 4050 DOI: 10.1039/D3QM00643C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements