Issue 14, 2021

Microfluidic generation of ATPS droplets by transient double emulsion technique

Abstract

An aqueous two-phase system (ATPS) is considered as a promising candidate for biological applications due to its excellent permeability, selective separation, rapid mass transfer and all-biocompatible nature. However, it remains difficult to generate ATPS emulsions with controllable and prolonged stability due to ultra-low interfacial tension. Here, we present a transient double emulsion (TDE) technique to address this challenge. The method involves two steps: (i) water-in-oil-in-water (W1/O/W2) transient double emulsion droplets are first produced with droplet microfluidics by introducing an additional middle oil phase, and (ii) the W1/O/W2 droplets dewet into oil-in-water and ATPS droplets in a controllable manner. Using the TDE method, both dextran-in-polyethylene glycol (PEG) and PEG-in-dextran ATPS droplets can be generated with high uniformity (coefficient of variation (CV) ranging from 0.66% to 2.55%), a wide range of droplet sizes (∼100 to 250 μm in radius), and tunability in the generation frequency (∼4 to 170 Hz). Tuning the oil viscosity controls the destabilization time for on-demand dewetting and releasing of aqueous core droplets. The stability of ATPS droplets is significantly improved by storing aqueous droplets in double emulsion vessels, which would benefit various applications, such as small molecule encapsulation.

Graphical abstract: Microfluidic generation of ATPS droplets by transient double emulsion technique

Supplementary files

Article information

Article type
Communication
Submitted
22 Сәу. 2021
Accepted
17 Мау. 2021
First published
18 Мау. 2021

Lab Chip, 2021,21, 2684-2690

Microfluidic generation of ATPS droplets by transient double emulsion technique

C. Zhou, P. Zhu, X. Han, R. Shi, Y. Tian and L. Wang, Lab Chip, 2021, 21, 2684 DOI: 10.1039/D1LC00351H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements