Issue 28, 2021

An integrating strategy for serum metabolomics and microarray analysis to expand the understanding of diet-induced obesity

Abstract

Obesity is a key component of metabolic syndrome and is precipitated by complex interactions between multiple environmental and genetic factors. The integration of multi-level bioinformation is needed to understand the altered endogenous molecule and metabolic mechanisms. In this study, an integrated analytical strategy was proposed by combining microarray data from a gene expression omnibus database and in vitro serum metabolomic data to unearth bioinformation associated with cafeteria diet induced obesity. In the diet induced obese rats, 23 genes and 9 metabolites showed significant changes, in which the increased levels of alanine, lactate and lactate dehydrogenase B (Ldhb) and the decreased levels of citrate and pyruvate indicated an enhanced glycolysis and a disordered Krebs cycle. Furthermore, the closeness centrality of Slc27a2, Apobr, alanine and histidine in the correlations network of pathways, genes and metabolites was 0.5036, 0.5111, 0.5702, and 0.5352, respectively. These close links between metabolites and genes would be highly useful to assess the degree of obesity and to understand the developmental mechanism of obesity. The pathway enrichment analysis of genes and metabolites proved that a disturbed glucose metabolism and biosynthesis of amino acids are typical metabolic features of cafeteria-induced obesity. The metabolomics combined with microarray data not only could identify the biomarkers, but also would be beneficial to the follow-up research of obesity treatment, especially providing a methodological basis for the study of other diseases.

Graphical abstract: An integrating strategy for serum metabolomics and microarray analysis to expand the understanding of diet-induced obesity

Supplementary files

Article information

Article type
Paper
Submitted
17 Мам. 2021
Accepted
15 Мау. 2021
First published
15 Мау. 2021

Anal. Methods, 2021,13, 3127-3135

An integrating strategy for serum metabolomics and microarray analysis to expand the understanding of diet-induced obesity

W. Liu, J. Xu, T. Dai, G. Shen and J. Feng, Anal. Methods, 2021, 13, 3127 DOI: 10.1039/D1AY00821H

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