Issue 12, 2021

A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils

Abstract

This report describes the development of a sensitive and broadly specific indirect competitive enzyme-linked immunosorbent assay (icELISA) and a gold nanoparticle-based immunochromatographic strip (GNP-ICS) assay for the detection of benzo[a]pyrene (B[a]P), using an anti-B[a]P monoclonal antibody (mAb). A broad-specific anti-B[a]P mAb (4E8) was raised from two types of haptens, with half maximal inhibitory concentrations and limits of detection (LOD) values of 2.51 and 0.54 ng mL−1, respectively. In addition, the cross-reactivity was up to 390% with structurally related compounds. The GNP-ICS assay based on a GNP-labeled mAb showed broad specificity in the detection of B[a]P and its analogues, with cut-off and visual LOD values of 100 and 10 ng mL−1, respectively. Furthermore, the recoveries from the developed icELISA and GNP-ICS assay in edible oil samples spiked with B[a]P were validated by high-performance liquid chromatography–fluorescence detection. The results revealed that the icELISA could reliably detect B[a]P in edible oils.

Graphical abstract: A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils

Supplementary files

Article information

Article type
Paper
Submitted
10 Сәу. 2021
Accepted
06 Мам. 2021
First published
06 Мам. 2021

Analyst, 2021,146, 3871-3879

A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils

J. Yao, X. Xu, L. Liu, H. Kuang, Z. Wang and C. Xu, Analyst, 2021, 146, 3871 DOI: 10.1039/D1AN00612F

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