Biodegradable microneedle patch for transdermal gene delivery

Moyuan Qu; Han-Jun Kim; Xingwu Zhou; Canran Wang; Xing Jiang; Jixiang Zhu; Yumeng Xue; Peyton Tebon; Shima A. Sarabi; Samad Ahadian; Mehmet R. Dokmeci; Songsong Zhu; Zhen Gu; Wujin Sun; Ali Khademhosseini

doi:10.1039/D0NR02759F

Biodegradable microneedle patch for transdermal gene delivery†

Moyuan Qu,^ab Han-Jun Kim,

^a Xingwu Zhou,^ac Canran Wang,^a Xing Jiang,^ad Jixiang Zhu,^ae Yumeng Xue,^a Peyton Tebon,

^a Shima A. Sarabi,^a Samad Ahadian,

^af Mehmet R. Dokmeci,

^af Songsong Zhu,^b Zhen Gu,

^ag Wujin Sun*^a and Ali Khademhosseini

*^acfgh

Author affiliations

* Corresponding authors

^a Department of Bioengineering, Center for Minimally Invasive Therapeutics, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA
E-mail: khademh@terasaki.org, wsun@terasaki.org

^b State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

^c Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA

^d School of Nursing, Nanjing University of Chinese Medicine, Nanjing 210023, China

^e Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, China

^f Department of Radiological Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA

^g Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA90095, USA

^h Terasaki Institute for Biomedical Innovation, Los Angeles, CA 90064, USA

Abstract

The skin houses a developed vascular and lymphatic network with a significant population of immune cells. Because of the properties of the skin, nucleic acid delivery through the tissue has the potential to treat a range of pathologies, including genetic skin conditions, hyperproliferative diseases, cutaneous cancers, wounds, and infections. This work presents a gelatin methacryloyl (GelMA) microneedle (MN)-based platform for local and controlled transdermal delivery of plasmid DNA (pDNA) with high transfection efficiency both in vitro and in vivo. Intracellular delivery of the nucleic acid cargo is enabled by poly(β-amino ester) (PBAE) nanoparticles (NPs). After being embedded in the GelMA MNs, sustained release of DNA-encapsulated PBAE NPs is achieved and the release profiles can be controlled by adjusting the degree of crosslinking of the GelMA hydrogel. These results highlight the advantages and potential of using PBAE/DNA NP-embedded GelMA MN patches (MN/PBAE/DNA) for successful transdermal delivery of pDNA for tissue regeneration and cancer therapy.

This article is part of the themed collection: Nanoscale Most Popular 2020 Articles

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Biodegradable microneedle patch for transdermal gene delivery†

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