Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

Lu Li; Lei Feng; Ming Zhang; Xin He; Shengqiao Luan; Chao Wang; Tony D. James; Houli Zhang; Huilian Huang; Xiaochi Ma

doi:10.1039/D0CC00197J

Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe†

Lu Li,‡^ab Lei Feng,

‡^bc Ming Zhang,‡^b Xin He,^b Shengqiao Luan,^b Chao Wang,

*^b Tony D. James,

*^d Houli Zhang,^b Huilian Huang^e and Xiaochi Ma

*^a

Author affiliations

* Corresponding authors

^a Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China
E-mail: maxiaochi2019@163.com

^b College of Pharmacy, Academy of Integrative Medicine, The National & Local Joint Engineering Research Center for Drug Development of Neurodegenerative Disease, Dalian Medical University, Dalian 116044, China
E-mail: wach_edu@sina.com

^c State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

^d Department of Chemistry, University of Bath, Bath BA2 7AY, UK
E-mail: t.d.james@bath.ac.uk

^e Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

Abstract

A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.

This article is part of the themed collection: The Mechanics of Supramolecular Chemistry

Chemical Communications

Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe†

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Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

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