Issue 10, 2020

Nanocomposites of poly(l-methionine), carbon nanotube–graphene complexes and Au nanoparticles on screen printed carbon electrodes for electrochemical analyses of dopamine and uric acid in human urine solutions

Abstract

A sensitive electrochemical sensor featuring novel composites of gold and carbon nanocomplexes alongside a polymerized amino acid was developed for the determination of uric acid (UA) and dopamine (DA) concentrations in both buffer and human urine sample solutions. The sensor was fabricated by electropolymerization of L-methionine (L-Met) followed by coating of carbon nanotube–graphene complexes and electrodeposition of gold nanoparticles on a screen printed carbon electrode surface. The electrode surfaces were characterized by field emission scanning electron microscopy and energy dispersive spectroscopy, and the electrochemical properties were investigated by cyclic voltammetry and differential pulse voltammetry. Linear ranges of 0.05–3 μM and 1–35 μM with limits of detection of 0.0029 and 0.034 μM were achieved for DA and UA, respectively. In addition, the developed sensor was applied for the analysis of native UA and DA concentrations in undiluted and diluted human urine samples. The UA analysis results were compared to those obtained using high performance liquid chromatography and a fluorometric assay kit while the DA analysis results were compared to those obtained using liquid chromatography-tandem mass spectrometry.

Graphical abstract: Nanocomposites of poly(l-methionine), carbon nanotube–graphene complexes and Au nanoparticles on screen printed carbon electrodes for electrochemical analyses of dopamine and uric acid in human urine solutions

Supplementary files

Article information

Article type
Paper
Submitted
31 Жел. 2019
Accepted
18 Ақп. 2020
First published
27 Ақп. 2020

Analyst, 2020,145, 3656-3665

Nanocomposites of poly(L-methionine), carbon nanotube–graphene complexes and Au nanoparticles on screen printed carbon electrodes for electrochemical analyses of dopamine and uric acid in human urine solutions

Y. Si, Y. E. Park, J. E. Lee and H. J. Lee, Analyst, 2020, 145, 3656 DOI: 10.1039/C9AN02638J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements