Issue 6, 2016

A highly specific graphene platform for sensing collagen triple helix

Abstract

The construction of simple and efficient assays to detect collagen biomarkers plays a critical role in developing novel diagnosis and therapies for the highly prevalent chronic fibroproliferative diseases. Inspired by the successful development of various GO-based biosensors for DNA utilizing its well-known double helix structure, we have for the first time created a highly specific GO platform for sensing the collagen triple helix. We have designed a dye-labeled single stranded collagen (ssCOL) peptide probe to target a complementary single stranded collagen peptide sequence. We have revealed that GO binds with the ssCOL probe and quenches the fluorescence of the dye, while the hybridization of the ssCOL probe with its target collagen peptide GPO results in the retention of the fluorescence of the probe. We have demonstrated that this design provides a fluorescence-enhanced assay that is sensitive and selective to the target collagen peptide with little interferences from other proteins, and it can be applied for quantitative detection in complex biological fluids. These results indicate that this GO-based ssCOL platform has great potential in molecular diagnostics of fibroproliferative diseases. It may provide a novel strategy to construct efficient assays for the proteins containing triple helix motifs such as collectins, adiponectin, macrophage scavenger and C1q.

Graphical abstract: A highly specific graphene platform for sensing collagen triple helix

Supplementary files

Article information

Article type
Paper
Submitted
23 Қаз. 2015
Accepted
22 Жел. 2015
First published
23 Жел. 2015

J. Mater. Chem. B, 2016,4, 1064-1069

A highly specific graphene platform for sensing collagen triple helix

X. Sun, J. Fan, W. Ye, H. Zhang, Y. Cong and J. Xiao, J. Mater. Chem. B, 2016, 4, 1064 DOI: 10.1039/C5TB02218E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements