Issue 4, 2016

Post-translational control of protein function with light using a LOV-intein fusion protein

Abstract

Methods for the post-translational control of protein function with light hold much value as tools in cell biology. To this end, we report a fusion protein that consists of DnaE split-inteins, flanking the light sensitive LOV2 domain of Avena sativa. The resulting chimera combines the activities of these two unrelated proteins to enable controlled formation of a functional protein via upregulation of intein splicing with blue light in bacterial and human cells.

Graphical abstract: Post-translational control of protein function with light using a LOV-intein fusion protein

Supplementary files

Article information

Article type
Paper
Submitted
04 Қаң. 2016
Accepted
01 Нау. 2016
First published
01 Нау. 2016

Mol. BioSyst., 2016,12, 1388-1393

Author version available

Post-translational control of protein function with light using a LOV-intein fusion protein

D. C. Jones, I. N. Mistry and A. Tavassoli, Mol. BioSyst., 2016, 12, 1388 DOI: 10.1039/C6MB00007J

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