Issue 11, 2015

In vivo histamine voltammetry in the mouse premammillary nucleus

Abstract

Histamine plays a major role in the mediation of allergic reactions such as peripheral inflammation. This classical monoamine is also a neurotransmitter involved in the central nervous system but its role in this context is poorly understood. Studying histamine neurotransmission is important due to its implications in many neurological disorders. The sensitivity, selectivity and high temporal resolution of fast scan cyclic voltammetry (FSCV) offer many advantages for studying electroactive neurotransmitters. Histamine has previously been studied with FSCV; however, the lack of a robust Faradaic electrochemical signal makes it difficult to selectively identify histamine in complex media, as found in vivo. In this work, we optimize an electrochemical waveform that provides a stimulation-locked and unique electrochemical signal towards histamine. We describe in vitro waveform optimization and a novel in vivo physiological model for stimulating histamine release in the mouse premammillary nucleus via stimulation of the medial forebrain bundle. We demonstrate that a robust signal can be used to effectively identify histamine and characterize its in vivo kinetics.

Graphical abstract: In vivo histamine voltammetry in the mouse premammillary nucleus

Article information

Article type
Paper
Submitted
15 Ақп. 2015
Accepted
07 Сәу. 2015
First published
08 Сәу. 2015

Analyst, 2015,140, 3759-3765

Author version available

In vivo histamine voltammetry in the mouse premammillary nucleus

S. Samaranayake, A. Abdalla, R. Robke, K. M. Wood, A. Zeqja and P. Hashemi, Analyst, 2015, 140, 3759 DOI: 10.1039/C5AN00313J

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