Issue 46, 2020

FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules

Abstract

In this article, we demonstrate fluorescent signal amplification via cyclic staining of target molecules (FRACTAL), a technique that can amplify the signal intensity of immunofluorescence staining more than nine-fold via simple cyclic staining of secondary antibodies. We also show that FRACTAL is compatible with four-color imaging and expansion microscopy imaging.

Graphical abstract: FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules

Supplementary files

Article information

Article type
Communication
Submitted
06 Там. 2020
Accepted
28 Қаз. 2020
First published
13 Қар. 2020

Nanoscale, 2020,12, 23506-23513

FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules

Y. Cho, J. Seo, Y. Sim, J. Chung, C. E. Park, C. G. Park, D. Kim and J. Chang, Nanoscale, 2020, 12, 23506 DOI: 10.1039/D0NR05800A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements