An acoustic levitation platform for high-content histological analysis of 3D tissue culture

Emilie Vuille-dit-Bille, Céline Loussert Fonta, Sarah Heub, Stéphanie Boder-Pasche, Mahmut Selman Sakar and Gilles Weder

Lab Chip, 2025, 25, 2732
DOI: 10.1039/D5LC00153F

Acoustofluidic assembly of 3D neurospheroids to model Alzheimer's disease

Hongwei Cai, Zheng Ao, Liya Hu, Younghye Moon, Zhuhao Wu, Hui-Chen Lu, Jungsu Kim and Feng Guo

Analyst, 2020, 145, 6243
DOI: 10.1039/D0AN01373K

On-chip hydrogel arrays individually encapsulating acoustic formed multicellular aggregates for high throughput drug testing

Xuejia Hu, Shukun Zhao, Ziyi Luo, Yunfeng Zuo, Fang Wang, Jiaomeng Zhu, Longfei Chen, Dongyong Yang, Yajing Zheng, Yujia Zheng, Yanxiang Cheng, Fuling Zhou and Yi Yang

Lab Chip, 2020, 20, 2228
DOI: 10.1039/D0LC00255K

Acoustic separation of living and dead cells using high density medium

Karl Olofsson, Björn Hammarström and Martin Wiklund

Lab Chip, 2020, 20, 1981
DOI: 10.1039/D0LC00175A

Acoustofluidics – changing paradigm in tissue engineering, therapeutics development, and biosensing

Reza Rasouli, Karina Martinez Villegas and Maryam Tabrizian

Lab Chip, 2023, 23, 1300
DOI: 10.1039/D2LC00439A

Spatiotemporal quantification of acoustic cell patterning using Voronoï tessellation

James P. K. Armstrong, Stephanie A. Maynard, Isaac J. Pence, Amanda C. Franklin, Bruce W. Drinkwater and Molly M. Stevens

Lab Chip, 2019, 19, 562
DOI: 10.1039/C8LC01108G

Scaffold-free generation of heterotypic cell spheroids using acoustofluidics

Zhuhao Wu, Bin Chen, Yue Wu, Yu Xia, Hui Chen, Zhiyi Gong, Hang Hu, Zhao Ding and Shishang Guo

Lab Chip, 2021, 21, 3498
DOI: 10.1039/D1LC00496D

Engineering the acoustic field with a Mie scatterer for microparticle patterning

Xingyu Jiang, Yunpeng Zhao, Minjie Shen, Xiao Zhou, Bin Chen, Bruce W. Drinkwater and Liangfei Tian

Lab Chip, 2025, 25, 413
DOI: 10.1039/D4LC00577E

Two-photon microscopy of acoustofluidic trapping for highly sensitive cell analysis

Thomas Kellerer, Bettina Sailer, Patrick Byers, Rune Barnkob, Oliver Hayden and Thomas Hellerer

Lab Chip, 2024, 24, 3456
DOI: 10.1039/D4LC00144C

A disposable acoustofluidic chip for nano/microparticle separation using unidirectional acoustic transducers

Shuaiguo Zhao, Mengxi Wu, Shujie Yang, Yuqi Wu, Yuyang Gu, Chuyi Chen, Jennifer Ye, Zhemiao Xie, Zhenhua Tian, Hunter Bachman, Po-Hsun Huang, Jianping Xia, Peiran Zhang, Heying Zhang and Tony Jun Huang

Lab Chip, 2020, 20, 1298
DOI: 10.1039/D0LC00106F

Fabrication of tunable, high-molecular-weight polymeric nanoparticles via ultrafast acoustofluidic micromixing

Shuaiguo Zhao, Po-Hsun Huang, Heying Zhang, Joseph Rich, Hunter Bachman, Jennifer Ye, Wenfen Zhang, Chuyi Chen, Zhemiao Xie, Zhenhua Tian, Putong Kang, Hai Fu and Tony Jun Huang

Lab Chip, 2021, 21, 2453
DOI: 10.1039/D1LC00265A

Research advances of acoustic particle manipulation techniques in field-assisted manufacturing

Jiaqi Wang, Xiaopeng Shang, Xinzhao Zhou and Huawei Chen

Nanoscale, 2025, 17, 5654
DOI: 10.1039/D4NR04891A

From Spheroids to Tumor-on-a-Chip for Cancer Modeling and Therapeutic Testing

Maria Veronica Lipreri, Marilina Tamara Totaro, Nicola Baldini and Sofia Avnet

Micromachines, 2025, 16, 1343
DOI: 10.3390/mi16121343

Accelerating particle aggregation in acoustic levitation by collective effects: Application to cost-effective ultrasonic harvesting of microalgae

Ludovic Bellebon, Michael Levant, Mauricio Hoyos and Jean-Luc Aider

Phys. Rev. Applied, 2025, 23
DOI: 10.1103/PhysRevApplied.23.014006

Ultrasound-induced reorientation for multi-angle optical coherence tomography

Mia Kvåle Løvmo, Shiyu Deng, Simon Moser, Rainer Leitgeb, Wolfgang Drexler and Monika Ritsch-Marte

Nat Commun, 2024, 15
DOI: 10.1038/s41467-024-46506-2

Ultrasonic manipulation in tissue engineering

Zijun Guan, Jianbo Huang, Yang Gao, Hongju Zhou, Liyun Wang, Lang Ma and Li Qiu

Engineered Regeneration, 2025, 6, 74
DOI: 10.1016/j.engreg.2025.03.001

An Overview on Spheroid and Organoid Models in Applied Studies

Zorislava Živković and Teuta Opačak-Bernardi

Sci, 2025, 7, 27
DOI: 10.3390/sci7010027

High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy

Steven J. Edwards, Valentina Carannante, Kyra Kuhnigk, Henrik Ring, Tatsiana Tararuk, Finn Hallböök, Hans Blom, Björn Önfelt and Hjalmar Brismar

Front. Mol. Biosci., 2020, 7
DOI: 10.3389/fmolb.2020.00208

Integrated Acoustic Chip for Culturing 3D Cell Arrays

Yong Luo, Hongxiao Gao, Mengyun Zhou, Long Xiao, Tailin Xu and Xueji Zhang

ACS Sens., 2022, 7, 2654
DOI: 10.1021/acssensors.2c01103

Conditional score-based diffusion models for solving inverse elasticity problems

Agnimitra Dasgupta, Harisankar Ramaswamy, Javier Murgoitio-Esandi, Ken Y. Foo, Runze Li, Qifa Zhou, Brendan F. Kennedy and Assad A. Oberai

Computer Methods in Applied Mechanics and Engineering, 2025, 433, 117425
DOI: 10.1016/j.cma.2024.117425

The waves that make the pattern: a review on acoustic manipulation in biomedical research

A.G. Guex, N. Di Marzio, D. Eglin, M. Alini and T. Serra

Materials Today Bio, 2021, 10, 100110
DOI: 10.1016/j.mtbio.2021.100110

Ultrasound-Responsive Systems as Components for Smart Materials

Athanasios G. Athanassiadis, Zhichao Ma, Nicolas Moreno-Gomez, Kai Melde, Eunjin Choi, Rahul Goyal and Peer Fischer

Chem. Rev., 2022, 122, 5165
DOI: 10.1021/acs.chemrev.1c00622

Sound innovations for biofabrication and tissue engineering

Mengxi Wu, Zhiteng Ma, Zhenhua Tian, Joseph T. Rich, Xin He, Jianping Xia, Ye He, Kaichun Yang, Shujie Yang, Kam W. Leong, Luke P. Lee and Tony Jun Huang

Microsyst Nanoeng, 2024, 10
DOI: 10.1038/s41378-024-00759-5

Strategies to control cellular spatial organization in microphysiological systems

Hung Dong Truong, Zhixing Ge, Elgene Chng, Y-Van Tran, Yusheng Zhang and Chwee Teck Lim

Microsyst Nanoeng, 2026, 12
DOI: 10.1038/s41378-025-01141-9

A Sessile Drop Method for Facile and Robust Spheroid Cultures

Bangyong Sun, Yi Zhao, Qiang Zhao and Gang Li

Adv Materials Inter, 2021, 8
DOI: 10.1002/admi.202100972

Acoustofluidics

James Friend

Front. Acoust., 2023, 1
DOI: 10.3389/facou.2023.1261027

Ultrasound-assisted tissue engineering

Kai Melde, Athanasios G. Athanassiadis, Dimitris Missirlis, Minghui Shi, Senne Seneca and Peer Fischer

Nat Rev Bioeng, 2024, 2, 486
DOI: 10.1038/s44222-024-00166-5

Characterization of red blood cell deformability induced by acoustic radiation force

Yifan Liu and Fengxian Xin

Microfluid Nanofluid, 2022, 26
DOI: 10.1007/s10404-021-02513-z

Large-scale high-throughput 3D culture, imaging, and analysis of cell spheroids using microchip-enhanced light-sheet microscopy

Tingting Zhu, Jun Nie, Tingting Yu, Dan Zhu, Yanyi Huang, Zaozao Chen, Zhongze Gu, Jiang Tang, Dongyu Li and Peng Fei

Biomed. Opt. Express, 2023, 14, 1659
DOI: 10.1364/BOE.485217

In vitro models to study natural killer cell dynamics in the tumor microenvironment

Valentina Carannante, Martin Wiklund and Björn Önfelt

Front. Immunol., 2023, 14
DOI: 10.3389/fimmu.2023.1135148

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

Pengchao Zhang, Ning Shao and Lidong Qin

Advanced Materials, 2021, 33
DOI: 10.1002/adma.202005944

High-throughput cell spheroid production and assembly analysis by microfluidics and deep learning

Martin Trossbach, Emma Åkerlund, Krzysztof Langer, Brinton Seashore-Ludlow and Haakan N. Joensson

SLAS Technology, 2023, 28, 423
DOI: 10.1016/j.slast.2023.03.003

Imaging Technologies for Microfluidic Biochips

Jeongmoo Han, Ungyo Kang, Eun-Yi Moon, Hongki Yoo and Bomi Gweon

BioChip J, 2022, 16, 255
DOI: 10.1007/s13206-022-00067-y

Scalable fabrication, compartmentalization and applications of living microtissues

Maik Schot, Nuno Araújo-Gomes, Bas van Loo, Tom Kamperman and Jeroen Leijten

Bioactive Materials, 2023, 19, 392
DOI: 10.1016/j.bioactmat.2022.04.005

Acoustic manipulation of multi-body structures and dynamics

Melody X Lim, Bryan VanSaders and Heinrich M Jaeger

Rep. Prog. Phys., 2024, 87, 064601
DOI: 10.1088/1361-6633/ad43f9

A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses

Hanna van Ooijen, Quentin Verron, Hanqing Zhang, Patrick A. Sandoz, Thomas W. Frisk, Valentina Carannante, Karl Olofsson, Arnika K. Wagner, Niklas Sandström and Björn Önfelt

Cell Reports Methods, 2025, 5, 100965
DOI: 10.1016/j.crmeth.2025.100965

A simple microsphere‐based mold to rapidly fabricate microwell arrays for multisize 3D tumor culture

Zixiu Li, Xiaofang Guo, Lili Sun, Juan Xu, Wenming Liu, Tianbao Li and Jinyi Wang

Biotech & Bioengineering, 2020, 117, 1092
DOI: 10.1002/bit.27257

Emerging biofabrication approaches for gastrointestinal organoids towards patient specific cancer models

Fernando Soto, Carlos F. Guimarães, Rui L. Reis, Walfre Franco, Imran Rizvi and Utkan Demirci

Cancer Letters, 2021, 504, 116
DOI: 10.1016/j.canlet.2021.01.023

Smart acoustic 3D cell construct assembly with high-resolution

Xuejia Hu, Jingjing Zheng, Qinghao Hu, Li Liang, Dongyong Yang, Yanxiang Cheng, Sen-Sen Li, Lu-Jian Chen and Yi Yang

Biofabrication, 2022, 14, 045003
DOI: 10.1088/1758-5090/ac7c90

Acoustofluidic separation enables early diagnosis of traumatic brain injury based on circulating exosomes

Zeyu Wang, Haichen Wang, Ryan Becker, Joseph Rufo, Shujie Yang, Brian E. Mace, Mengxi Wu, Jun Zou, Daniel T. Laskowitz and Tony Jun Huang

Microsyst Nanoeng, 2021, 7
DOI: 10.1038/s41378-021-00244-3

Ultrasound-Based Scaffold-Free Core-Shell Multicellular Tumor Spheroid Formation

Karl Olofsson, Valentina Carannante, Madoka Takai, Björn Önfelt and Martin Wiklund

Micromachines, 2021, 12, 329
DOI: 10.3390/mi12030329

From cell spheroids to vascularized cancer organoids: Microfluidic tumor-on-a-chip models for preclinical drug evaluations

Yue Wu, Yuyuan Zhou, Xiaochen Qin and Yaling Liu

Biomicrofluidics, 2021, 15
DOI: 10.1063/5.0062697

A Novel Microfluidic Platform for Personalized Anticancer Drug Screening Through Image Analysis

Maria Veronica Lipreri, Marilina Tamara Totaro, Julia Alicia Boos, Maria Sofia Basile, Nicola Baldini and Sofia Avnet

Micromachines, 2024, 15, 1521
DOI: 10.3390/mi15121521

Adaptive ultrasonic actuation for dynamic formation and characterization of 3D cell cultures

Björn Hammarström, Karl Olofsson, Valentina Carannante, Sarah Alberio, Patrick A. Sandoz, Björn Önfelt and Martin Wiklund

Sensors and Actuators B: Chemical, 2025, 427, 137173
DOI: 10.1016/j.snb.2024.137173

Dynamic cell patterning and photopolymerization with electric field modulation for constructing hierarchical tumor microenvironments

Anping Wu, Yanfeng Zhao, Xinyi Dong, Jiaxin Liu, Zhiqiang Zheng, Qing Shi, Toshio Fukuda and Huaping Wang

Acta Biomaterialia, 2026, 209, 339
DOI: 10.1016/j.actbio.2025.11.020

Cell Spheroid Formation on the Surface of Multi‐Block Copolymers Composed of Poly(2‐methoxyethyl acrylate) and Polyethylene Glycol

Naoto Ogiwara, Takenobu Nakano, Tsukuru Masuda, Keiichiro Kushiro and Madoka Takai

Macromolecular Bioscience, 2023, 23
DOI: 10.1002/mabi.202200486

Acoustic Focusing of Protein Crystals for In-Line Monitoring and Up-Concentration during Serial Crystallography

Björn Hammarström, Thomas J. Lane, Hazal Batili, Raymond Sierra, Martin Wiklund and Jonas A. Sellberg

Anal. Chem., 2022, 94, 12645
DOI: 10.1021/acs.analchem.2c01701

Acoustophoretic particle motion in a spherical microchamber

Bettina Sailer, Rune Barnkob and Oliver Hayden

Phys. Rev. Applied, 2024, 22
DOI: 10.1103/PhysRevApplied.22.044034

Recent advances in microfluidic technologies for organ-on-a-chip

Chang Tian, Qin Tu, Wenming Liu and Jinyi Wang

TrAC Trends in Analytical Chemistry, 2019, 117, 146
DOI: 10.1016/j.trac.2019.06.005

Assembling Living Building Blocks to Engineer Complex Tissues

Liliang Ouyang, James P. K. Armstrong, Manuel Salmeron‐Sanchez and Molly M. Stevens

Adv Funct Materials, 2020, 30
DOI: 10.1002/adfm.201909009

A superhydrophobic chip integrated with an array of medium reservoirs for long-term hanging drop spheroid culture

Bangyong Sun, Yi Zhao, Weimin Wu, Qiang Zhao and Gang Li

Acta Biomaterialia, 2021, 135, 234
DOI: 10.1016/j.actbio.2021.08.006

Acoustofluidics for biomedical applications

Joseph Rufo, Feiyan Cai, James Friend, Martin Wiklund and Tony Jun Huang

Nat Rev Methods Primers, 2022, 2
DOI: 10.1038/s43586-022-00109-7

Acoustofluidics for cell patterning and tissue engineering

Zhuhao Wu, Meidie Pan, Jinglin Wang, Baojie Wen, Ling Lu and Haozhen Ren

Engineered Regeneration, 2022, 3, 397
DOI: 10.1016/j.engreg.2022.08.005

Imaging‐Based Efficacy Evaluation of Cancer Immunotherapy in Engineered Tumor Platforms and Tumor Organoids

Seong‐Eun Kim, Suji Yun, Junsang Doh and Hong Nam Kim

Adv Healthcare Materials, 2024, 13
DOI: 10.1002/adhm.202400475

A customizable micropatterned platform for osteosarcoma spheroid generation, imaging, and drug screening

Maria Veronica Lipreri, Marilina Tamara Totaro, Ilaria Raimondi, Margherita Cortini, Nicola Baldini and Sofia Avnet

Biomaterials Advances, 2025, 177, 214419
DOI: 10.1016/j.bioadv.2025.214419

Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip

Niklas Sandström, Valentina Carannante, Karl Olofsson, Patrick A. Sandoz, Elisabeth L. Moussaud-Lamodière, Brinton Seashore-Ludlow, Hanna Van Ooijen, Quentin Verron, Thomas Frisk, Madoka Takai, Martin Wiklund, Päivi Östling and Björn Önfelt

Cell Reports Methods, 2022, 2, 100256
DOI: 10.1016/j.crmeth.2022.100256

Ultrasonic Based Tissue Modelling and Engineering

Karl Olofsson, Björn Hammarström and Martin Wiklund

Micromachines, 2018, 9, 594
DOI: 10.3390/mi9110594

Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids

Karl Olofsson, Valentina Carannante, Madoka Takai, Björn Önfelt and Martin Wiklund

Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-96288-6