Novel TICT-characterized fluorescent probes for efficient imaging of lipid droplets in nonalcoholic fatty liver disease

Abstract

Dysfunction of lipid droplets (LDs) is closely associated with nonalcoholic fatty liver disease (NAFLD). Although fluorescence imaging has the advantages of noninvasiveness and high spatiotemporal resolution in the detection of LDs, the imaging performance of existing commercial fluorescent probes is still unsatisfactory. In this study, we developed two novel fluorescent probes, DAB-LD and DAI-LD, featuring twisted intramolecular charge transfer (TICT) properties for efficient LD-specific imaging. These probes exhibit minimal fluorescence in aqueous solutions but significantly enhanced emission in lipid environments, indicating good photophysical properties and biocompatibility. Moreover, both of these probes specifically target intracellular LDs and exhibit excellent imaging performance. Importantly, DAI-LD has shown effective imaging capabilities in a mouse model of NAFLD. This study not only provides a robust tool for NAFLD research but also introduces a new strategy for designing LDs-specific fluorescent probes.

Graphical abstract: Novel TICT-characterized fluorescent probes for efficient imaging of lipid droplets in nonalcoholic fatty liver disease

Supplementary files

Article information

Article type
Paper
Submitted
26 მარ 2025
Accepted
17 მაი 2025
First published
06 ივნ 2025

Anal. Methods, 2025, Advance Article

Novel TICT-characterized fluorescent probes for efficient imaging of lipid droplets in nonalcoholic fatty liver disease

D. Yang, T. Qu, L. Teng, C. Wang, W. Bai, S. Li, W. Zhuang and W. Hu, Anal. Methods, 2025, Advance Article , DOI: 10.1039/D5AY00499C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements