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The solvent requirements for an organic reaction are different from those of electrospray mass spectrometry (ESI-MS) analysis; therefore, some reaction solutions in non-ESI friendly solvents cannot be directly studied using ESI-MS. This mini account discusses some of our recent contributions in solving the challenging problems in the characterization of reactive intermediates in reaction solutions in non-ESI friendly solvents by using ESI-based mass spectrometry.

Graphical abstract: The remarkable role of solvent in reaction mechanism studies by electrospray mass spectrometry

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