Single-labeled peptide substrates for detection of protease activity based on the inherent fluorescence quenching ability of Cu2+

Dehua Deng, Yuanqiang Hao, Ping Yang, Ning Xia, Wenxue Yu, Xiuhua Liu and Lin Liu

Anal. Methods, 2019, 11, 1248
DOI: 10.1039/C8AY02650E

PET and FRET utility of an amino acid pair: tryptophan and 4-cyanotryptophan

Ismail A. Ahmed, Jeffrey M. Rodgers, Christina Eng, Thomas Troxler and Feng Gai

Phys. Chem. Chem. Phys., 2019, 21, 12843
DOI: 10.1039/C9CP02126D

Perspective on chymotrypsin detection

Haixia Shi, Cheng Liu, Jingjie Cui, Jia Cheng, Yuanwei Lin, Li Gao and Rong Luo

New J. Chem., 2020, 44, 20921
DOI: 10.1039/D0NJ04454G

Ultrasensitive and highly selective FRET aptasensor for Hg2+ measurement in fish samples using carbon dots/AuNPs as donor/acceptor platform

Shole Amiri, Rezgar Ahmadi, Abdollah Salimi, Aso Navaee, Somaye Hamd Qaddare and Mohammad Kazem Amini

New J. Chem., 2018, 42, 16027
DOI: 10.1039/C8NJ02781A

A new multi-purpose FRET fluorescent probe for the simultaneous detection of proteases

Petra Jedináková, Zuzana Barbuščáková, David Milićević, Martin Kubala and Jan Hlaváč

RSC Adv., 2025, 15, 34821
DOI: 10.1039/D5RA03163J

Integration of a peptide–DNA conjugate with multiple cyclic signal amplification for the ultrasensitive detection of cathepsin B activity

Zi-yue Wang, Cheng-peng Zhang and Chun-yang Zhang

Chem. Commun., 2020, 56, 2119
DOI: 10.1039/C9CC09714G

Peptide probes for proteases – innovations and applications for monitoring proteolytic activity

Maria Rodriguez-Rios, Alicia Megia-Fernandez, Daniel J. Norman and Mark Bradley

Chem. Soc. Rev., 2022, 51, 2081
DOI: 10.1039/D1CS00798J

A protein-nanoparticle conjugate platform for simplified and sensitive protease activity assays

Masafumi Sakono, Kazuki Higashi, Mitsuki Nakamura and Naomi Sakono

Anal. Methods, 2025, 17, 7896
DOI: 10.1039/D5AY01006C

Miniaturisation of a peptide-based electrochemical protease activity sensor using platinum microelectrodes

Ahmet Ucar, Eva González-Fernández, Matteo Staderini, Nicolaos Avlonitis, Alan F. Murray, Mark Bradley and Andrew R. Mount

Analyst, 2020, 145, 975
DOI: 10.1039/C9AN02321F

An upconversion nanoparticle-based fluorescence resonance energy transfer system for effectively sensing caspase-3 activity

Lin Liu, Hua Zhang, Daqian Song and Zhenxin Wang

Analyst, 2018, 143, 761
DOI: 10.1039/C7AN01744H

Peptomer substrates for quantitative pattern-recognition sensing of proteases

Mariah J. Austin, Hattie C. Schunk, Natalie Ling and Adrianne M. Rosales

Chem. Commun., 2023, 59, 1685
DOI: 10.1039/D2CC06587H

An anti-interference near-infrared SERS tag-aptamer sensor for rapid detection of Staphylococcus aureus in complex matrices

Rui Zhao, Qishuo Wang, Yunqing Wang, Rongchao Mei, Xiaoyan Wang and Lingxin Chen

Analyst, 2026, 151, 1495
DOI: 10.1039/D5AN01324K

An antibody-based amperometric biosensor for 20S proteasome activity and inhibitor screening

Madalina M. Barsan and Victor C. Diculescu

Analyst, 2021, 146, 3216
DOI: 10.1039/D0AN02426K

Single-Walled Carbon Nanotube Probes for Protease Characterization Directly in Cell-Free Expression Reactions

Sepehr Hejazi, Ryan Godin, Vito Jurasic and Nigel F. Reuel

Anal. Chem., 2025, 97, 10745
DOI: 10.1021/acs.analchem.5c00883

Sensitive Affinity-Based Biosensor Using the Autocatalytic Activation of Trypsinogen Mutant by Trypsin with Low Self-activation

Seonhwa Park, Hyoeun Lee and Haesik Yang

ACS Appl. Bio Mater., 2022, 5, 4516
DOI: 10.1021/acsabm.2c00594

Bio-barcode assay: A useful technology for ultrasensitive and logic-controlled specific detection in food safety: A review

Yue Hou, Ruipeng Chen, Zhiguang Wang, Ran Lu, Yonghui Wang, Shuyue Ren, Shuang Li, Yu Wang, Tie Han, Shiping Yang, Huanying Zhou and Zhixian Gao

Analytica Chimica Acta, 2023, 1267, 341351
DOI: 10.1016/j.aca.2023.341351

A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection

Masashi Arakawa, Akiho Yoshida, Shinya Okamura, Hirotaka Ebina and Eiji Morita

Sci Rep, 2023, 13
DOI: 10.1038/s41598-023-28984-4

Stable isotope-labeled paper spray mass spectrometry enables high-sensitivity profiling of protease activity

Zhiqing Zhou, Weiqing Wang, Xiunan Zhai, Miaomiao Qin, Zhao Wang and Yanmei Yang

Talanta, 2026, 297, 128736
DOI: 10.1016/j.talanta.2025.128736

A Metal–Organic Hybrid Composed of Dual Quenching Cofactors as a Nanoquencher for the Fluorescent Determination of Protease Caspase-3

Fengli Gao, Lin Liu, Cancan He, Yong Chang and Weiqiang Wang

Biosensors, 2025, 15, 354
DOI: 10.3390/bios15060354

Novel Detection Method for Evaluating the Activity of an Alkaline Serine Protease from Bacillus clausii

Xinyue Li, Shaodong Ren, Guangchao Song, Yihan Liu, Yu Li and Fuping Lu

J. Agric. Food Chem., 2022, 70, 3765
DOI: 10.1021/acs.jafc.2c00358

Electrochemical Activity Assay for Protease Analysis Using Carbon Nanofiber Nanoelectrode Arrays

Yang Song, Huafang Fan, Morgan J. Anderson, Jestin Gage Wright, Duy H. Hua, Jessica Koehne, M. Meyyappan and Jun Li

Anal. Chem., 2019, 91, 3971
DOI: 10.1021/acs.analchem.8b05189

Detection of Gingipain Activity Using Solid State Nanopore Sensors

Quentin Palomar, Daniel Aili, Zhen Zhang, Anna Svärd, Qitao Hu, Funing Liu and Shuangshuang Zeng

SSRN Journal, 2022
DOI: 10.2139/ssrn.4066369

Activator Strand Modifications in CRISPR/Cas12a: Unlocking the Potential for Casp-3-Targeted Biosensing and Imaging Analysis of Apoptosis

Qing-Nan Li, Yun-Xi Cui, Zhi-Qi Dai, Zhi-Li Yao, Man-Ying Li, Qi-Liang Cai and De-Ming Kong

Anal. Chem., 2025, 97, 4194
DOI: 10.1021/acs.analchem.4c06591

Diagnostic and therapeutic potential of protease inhibition

Natalia Ćwilichowska, Karolina W. Świderska, Agnieszka Dobrzyń, Marcin Drąg and Marcin Poręba

Molecular Aspects of Medicine, 2022, 88, 101144
DOI: 10.1016/j.mam.2022.101144

Quantitative Detection of Cathepsin B Activity in Neutral pH Buffers Using Gold Microelectrode Arrays: Toward Direct Multiplex Analyses of Extracellular Proteases in Human Serum

Yang Song, Jestin Gage Wright, Morgan J. Anderson, Sabari Rajendran, Zhaoyang Ren, Duy H. Hua, Jessica E. Koehne, M. Meyyappan and Jun Li

ACS Sens., 2021, 6, 3621
DOI: 10.1021/acssensors.1c01175

Rapid and accurate quantification of trypsin activity using integrated infrared and ultraviolet spectroscopy with data fusion techniques

Wen-Xiu Zhi, Bao-Rong Wang, Jie Zhou, Ying-Chao Qiu, Si-Yu Lu, Jing-Zhi Yu, Ying-Hua Zhang and Zhi-Shen Mu

International Journal of Biological Macromolecules, 2024, 278, 135017
DOI: 10.1016/j.ijbiomac.2024.135017

Electrochemical biosensor for trypsin activity assay based on cleavage of immobilized tyrosine-containing peptide

Tatiana A. Filippova, Rami A. Masamrekh, Victoria V. Shumyantseva, Ivan A. Latsis, Tatiana E. Farafonova, Irina Y. Ilina, Sergey L. Kanashenko, Sergei A. Moshkovskii and Alexey V. Kuzikov

Talanta, 2023, 257, 124341
DOI: 10.1016/j.talanta.2023.124341

The role of fungal proteases in bioactive peptide production and analytical approaches for tracking their activity

Tanvir Ahmed, Angéla Juhász, Utpal Bose, Netsane Shiferaw Terefe and Michelle L. Colgrave

TrAC Trends in Analytical Chemistry, 2025, 192, 118359
DOI: 10.1016/j.trac.2025.118359

Utilizing Two Electrokinetic Techniques on a Single Device for Detection of Extracellular Vesicle‐Associated Protease Activity

Ella Stimson, Delaney Shea, Michelle M. Gomes, Srivathsan Ranganathan, Sarah Mitchell, Jason Ware, Randall Armstrong, Christian Ross, Michael Heller and Stuart D. Ibsen

Small, 2025, 21
DOI: 10.1002/smll.202404457

A Matrix Metalloproteinase Sensing Biosensor for the Evaluation of Chronic Wounds

So Min Kang, Hana Cho, Dasom Jeon, Seong Hyuk Park, Dong-Sik Shin and Chan Yeong Heo

BioChip J, 2019, 13, 323
DOI: 10.1007/s13206-019-3403-4

GC-MS, LC-MS/MS, Docking and Molecular Dynamics Approaches to Identify Potential SARS-CoV-2 3-Chymotrypsin-Like Protease Inhibitors from Zingiber officinale Roscoe

Muhammad Zubair, Saipul Maulana, Agustinus Widodo, Ramadanil Pitopang, Muhammad Arba and Maywan Hariono

Molecules, 2021, 26, 5230
DOI: 10.3390/molecules26175230

Interfacial behavior of alkaline protease at the air-water and oil-water interfaces

Jian Zhang, Yanyan Li, Jing Wang and Yue Zhang

Applied Surface Science, 2018, 433, 1128
DOI: 10.1016/j.apsusc.2017.09.238

Remote biosensor for the determination of trypsin by using nanoporous anodic alumina as a three-dimensional nanostructured material

Mahmoud Amouzadeh Tabrizi, Josep Ferré-Borrull and Lluis F. Marsal

Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-59287-7

Magnetically-assisted electrochemical immunoplatform for simultaneous detection of active and total prostate-specific antigen based on proteolytic reaction and sandwich affinity analysis

Ning Xia, Gang Liu, Yonghong Chen, Tong Wu, Lin Liu, Suling Yang and Yuanyuan Li

Talanta, 2024, 270, 125534
DOI: 10.1016/j.talanta.2023.125534

A Charge‐Switchable Zwitterionic Peptide for Rapid Detection of SARS‐CoV‐2 Main Protease

Zhicheng Jin, Yash Mantri, Maurice Retout, Yong Cheng, Jiajing Zhou, Alec Jorns, Pavla Fajtova, Wonjun Yim, Colman Moore, Ming Xu, Matthew N. Creyer, Raina M. Borum, Jingcheng Zhou, Zhuohong Wu, Tengyu He, William F. Penny, Anthony J. O'Donoghue and Jesse V. Jokerst

Angew Chem Int Ed, 2022, 61
DOI: 10.1002/anie.202112995

Novel biosensor platforms for the detection of coronavirus infection and severe acute respiratory syndrome coronavirus 2

Kung-Hao Liang, Tai-Jay Chang, Mong-Lien Wang, Ping-Hsing Tsai, Ta-Hsien Lin, Chin-Tien Wang and De-Ming Yang

Journal of the Chinese Medical Association, 2020, 83, 701
DOI: 10.1097/JCMA.0000000000000337

A Brain-Targeting Nanoprobe for On-Site BACE1 Detection and Reversal of Microglia-Derived Neuroinflammation

Xinyue Liu, Ruijie Cao, Yijing Dang, Zhiai Xu and Wen Zhang

ACS Appl. Nano Mater., 2025, 8, 6479
DOI: 10.1021/acsanm.5c00123

Matrix Metalloproteinase-9 (MMP-9) Activatable Gold Nanoparticles for In Situ Zymography and Diagnostics of Neurofibromatosis Type 2 (NF2) Tumors

Shimayali Kaushal, Han T. N. Nguyen, Melanie Fisher, Hsuan-Chih Kuo, Zachary D. Schultz and Yin Ren

ACS Appl. Nano Mater., 2026, 9, 2245
DOI: 10.1021/acsanm.5c04657

Enzyme-Instructed Formation of β-Sheet-Rich Nanoplatelets for Label-Free Protease Sensing

Emma Olson, Justin S. Plaut, Sarah A. Barnhill, Sinan Sabuncu, Corey M. Dambacher, Sean D. Speese, Srivathsan V. Ranganathan, Bruce P. Branchaud and Adem Yildirim

ACS Appl. Nano Mater., 2021, 4, 7800
DOI: 10.1021/acsanm.1c01098

Natural-Target-Mimicking Translocation-Based Fluorescent Sensor for Detection of SARS-CoV-2 PLpro Protease Activity and Virus Infection in Living Cells

Elena L. Sokolinskaya, Olga N. Ivanova, Irina T. Fedyakina, Alexander V. Ivanov and Konstantin A. Lukyanov

IJMS, 2024, 25, 6635
DOI: 10.3390/ijms25126635

Small Surface, Big Effects, and Big Challenges: Toward Understanding Enzymatic Activity at the Inorganic Nanoparticle–Substrate Interface

W. Russ Algar, Tiffany Jeen, Melissa Massey, William J. Peveler and Jérémie Asselin

Langmuir, 2019, 35, 7067
DOI: 10.1021/acs.langmuir.8b02733

Development of Small-Molecule Fluorescent Probes Targeting Enzymes

Yuan-Xiang Li, Dong-Tai Xie, Ya-Xi Yang, Zhao Chen, Wu-Yingzheng Guo and Wen-Chao Yang

Molecules, 2022, 27, 4501
DOI: 10.3390/molecules27144501

Equipment-free determination of trypsin and its inhibitor based on the distance-readout via viscosity change on paper substrates

Xiaoxia Liu, Fanghua Xia, Guojuan Zhang, Wenmei Gao, Xiaodi Guo, Jinzhong Zhao, Wuyong Zhao and Miaomiao Tian

Microchemical Journal, 2026, 220, 116463
DOI: 10.1016/j.microc.2025.116463

Simultaneous, multiplex quantification of protease activities using a gold microelectrode array

Morgan J. Anderson, Yang Song, Huafang Fan, Jestin Gage Wright, Zhaoyang Ren, Duy H. Hua, Jessica E. Koehne, M. Meyyappan and Jun Li

Biosensors and Bioelectronics, 2020, 165, 112330
DOI: 10.1016/j.bios.2020.112330

Enzyme-free and label-free detection of Staphylococcus aureus based on target-inhibited fluorescence signal recovery

Shuo Yao, Chao Zhao, Mingyu Shang, Juan Li and Juan Wang

Food and Chemical Toxicology, 2021, 150, 112071
DOI: 10.1016/j.fct.2021.112071

Giant magnetoresistive biosensors for real-time quantitative detection of protease activity

Sandeep Adem, Sonal Jain, Michael Sveiven, Xiahan Zhou, Anthony J. O’Donoghue and Drew A. Hall

Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-62910-2

Visualization of Ectopic Serine Protease Activity by Förster Resonance Energy Transfer-Based Reporters

Verena Rickert-Zacharias, Madeleine Schultz, Marcus A. Mall and Carsten Schultz

ACS Chem. Biol., 2021, 16, 2174
DOI: 10.1021/acschembio.1c00168

Selective Detection of Active Extracellular Granzyme A by Using a Novel Fluorescent Immunoprobe with Application to Inflammatory Diseases

Ana Senan-Salinas, Laura Comas, Patricia Esteban, Marcela Garzón-Tituaña, Zhiming Cheng, Llipsy Santiago, Maria Pilar Domingo, Ariel Ramírez-Labrada, José Ramón Paño-Pardo, Marc Vendrell, Julián Pardo, Maykel A. Arias and Eva M. Galvez

ACS Pharmacol. Transl. Sci., 2024, 7, 1474
DOI: 10.1021/acsptsci.4c00065

Imidazopyridines as fluorogenic substrates for esterase detection

Chiara Reviglio, Giorgio Volpi, Elisabeth Wyart, Betty Ciubini, Cristina Prandi, Claudia Barolo, Paolo Ettore Porporato and Claudio Garino

Journal of Photochemistry and Photobiology A: Chemistry, 2025, 462, 116256
DOI: 10.1016/j.jphotochem.2024.116256

A fluorogenic, peptide-based probe for the detection of Cathepsin D in macrophages

Maria Rodriguez-Rios, Brian J. McHugh, Zhengqi Liang, Alicia Megia-Fernandez, Annamaria Lilienkampf, David Dockrell and Mark Bradley

Commun Chem, 2023, 6
DOI: 10.1038/s42004-023-01035-9

Enzymatic reaction modulated synthesis of quantum dots for visual detection of cholinesterase activity and inhibitor

Wenqing Deng, Piaopiao Chen, Pingyue Hu, Ziying He, Mei Zhang, Xin Yuan and Ke Huang

Sensors and Actuators B: Chemical, 2019, 292, 180
DOI: 10.1016/j.snb.2019.04.136

Protease detection in the biosensor era: A review

Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe and Alexey Tarasov

Biosensors and Bioelectronics, 2024, 244, 115788
DOI: 10.1016/j.bios.2023.115788

Synthetic peptide array on gold for the electrochemical assessment of the 20S proteasome activity and effect of inhibitory compounds

Madalina M. Barsan, Ariana Serban, Melania Onea, Magdalena Wysocka, Adam Lesner and Victor C. Diculescu

Applied Surface Science, 2023, 610, 155620
DOI: 10.1016/j.apsusc.2022.155620

Coupling hCG-based protease sensors with a commercial pregnancy test strip for simple analyses of protease activities

Hyeon Ji Park, Yuseon Kim, Kyung Won Lee, Minji Gwon, Hyun C. Yoon and Tae Hyeon Yoo

Biosensors and Bioelectronics, 2023, 235, 115364
DOI: 10.1016/j.bios.2023.115364

Trypsin Detection Using Electrochemical Reduction‐based Redox Cycling

Jeonghwa Shin, Kiryeon Park, Seonhwa Park and Haesik Yang

Bulletin Korean Chem Soc, 2021, 42, 37
DOI: 10.1002/bkcs.12147

Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors

Jing Zhang, Dan Xu, Zijie Deng, Xueping Tan, Dongnan Guo, Yanru Qiao, You Li, Xiaofang Hou, Sicen Wang and Junbo Zhang

Talanta, 2024, 267, 125267
DOI: 10.1016/j.talanta.2023.125267

Identification of SARS-CoV-2 Main Protease Cleavage Sites in Bovine β-Casein

János András Mótyán, Tibor Nagy, Ágota Nagyné Veres, Mária Golda, Mohamed Mahdi and József Tőzsér

IJMS, 2025, 26, 5829
DOI: 10.3390/ijms26125829

Chronoamperometric Biosensor for Protease Activity Assay and Inhibitor Screening

Loubna El Harrad and Aziz Amine

Electroanalysis, 2017, 29, 2395
DOI: 10.1002/elan.201700340

A Paper-Based Near-Infrared Optical Biosensor for Quantitative Detection of Protease Activity Using Peptide-Encapsulated SWCNTs

Vlad Shumeiko, Yossi Paltiel, Gili Bisker, Zvi Hayouka and Oded Shoseyov

Sensors, 2020, 20, 5247
DOI: 10.3390/s20185247

Voltammetric sensing of trypsin activity using gelatin as a substrate

Noemi Poma, Federico Vivaldi, Andrea Bonini, Denise Biagini, Daria Bottai, Arianna Tavanti and Fabio Di Francesco

Microchemical Journal, 2023, 190, 108631
DOI: 10.1016/j.microc.2023.108631

A Molecular Probe with Both Chromogenic and Fluorescent Units for Detecting Serine Proteases

Kirara Ishida, Yushi Nakamura, Tetsuo Ohta and Yohei Oe

Molecules, 2021, 26, 482
DOI: 10.3390/molecules26020482

Gadolinium-Cyclic 1,4,7,10-Tetraazacyclododecane-1,4,7,10-Tetraacetic Acid-Click-Sulfonyl Fluoride for Probing Serine Protease Activity in Magnetic Resonance Imaging

Phuong Tu Huynh, Huy Duc Vu, Junghwa Ryu, Hee Su Kim, Hoesu Jung and Sung Won Youn

Molecules, 2023, 28, 3538
DOI: 10.3390/molecules28083538

Study on the interaction between calcium ions and alkaline protease of bacillus

Jian Zhang, Jing Wang, Yongxiang Zhao, Jing Li and Yaoming Liu

International Journal of Biological Macromolecules, 2019, 124, 121
DOI: 10.1016/j.ijbiomac.2018.11.198

Proteolytic Biosensors with Functional Nanomaterials: Current Approaches and Future Challenges

Jin-Ha Choi

Biosensors, 2023, 13, 171
DOI: 10.3390/bios13020171

Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle

Xiaohua Ma, Yingxin Lv, Panpan Liu, Yuanqiang Hao and Ning Xia

Molecules, 2023, 28, 3426
DOI: 10.3390/molecules28083426

Electrochemical Protein-based Bioanalytical Devices for Drug Analysis

Caroline G. Sanz and Victor C. Diculescu

CTMC, 2023, 23, 1448
DOI: 10.2174/1568026623666230411152640

Electrochemical sensing interface based on the oriented self-assembly of histidine labeled peptides induced by Ni2+ for protease detection

Fei Wang, Yichen Gong, Yang Xu, Zhanfang Ma and Hongliang Han

Biosensors and Bioelectronics, 2023, 230, 115259
DOI: 10.1016/j.bios.2023.115259

Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection

David Milićević and Jan Hlaváč

Chemosensors, 2021, 9, 119
DOI: 10.3390/chemosensors9060119

De Novo Design of a Highly Selective Nonpeptide Fluorogenic Probe for Chymotrypsin Activity Sensing in a Living System

Shi-Yu Liu, Xiaoting Zou, Xing Gao and Yue-Yang Zhang

Anal. Chem., 2022, 94, 17922
DOI: 10.1021/acs.analchem.2c03933

Liquid crystal-enabled protease inhibition assays developed in a millifluidic device

Mahbuba Jannat and Kun-Lin Yang

Sensors and Actuators B: Chemical, 2019, 296, 126595
DOI: 10.1016/j.snb.2019.05.072

Ratiometric fluorescent detection of protease activity in foods based on microwave-assisted synthesized casein-directed gold nanoclusters

Haoxing You, Jun Xiong, Ruichen Gao, Wen-Yong Lou and Xiaoling Wu

Food Chemistry, 2025, 474, 143078
DOI: 10.1016/j.foodchem.2025.143078

A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

Yunxiao Feng, Gang Liu, Fan Zhang, Jianwen Liu, Ming La and Ning Xia

Micromachines, 2022, 13, 803
DOI: 10.3390/mi13050803

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Mark Dizon, Marek Tatarko and Tibor Hianik

Sensors, 2020, 20, 5594
DOI: 10.3390/s20195594

Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids

Nina Turk, Ali Raza, Pieter Wuytens, Hans Demol, Michiel Van Daele, Christophe Detavernier, Andre Skirtach, Kris Gevaert and Roel Baets

Biomed. Opt. Express, 2020, 11, 4800
DOI: 10.1364/BOE.398038

The multifaceted role of proteases and modern analytical methods for investigation of their catalytic activity

Tatiana A. Filippova, Rami A. Masamrekh, Yulia Yu. Khudoklinova, Victoria V. Shumyantseva and Alexey V. Kuzikov

Biochimie, 2024, 222, 169
DOI: 10.1016/j.biochi.2024.03.006

Acoustic biosensors for ultrasound imaging of enzyme activity

Anupama Lakshmanan, Zhiyang Jin, Suchita P. Nety, Daniel P. Sawyer, Audrey Lee-Gosselin, Dina Malounda, Mararet B. Swift, David Maresca and Mikhail G. Shapiro

Nat Chem Biol, 2020, 16, 988
DOI: 10.1038/s41589-020-0591-0

Design and Application of Sensors for Chemical Cytometry

Brianna M. Vickerman, Matthew M. Anttila, Brae V. Petersen, Nancy L. Allbritton and David S. Lawrence

ACS Chem. Biol., 2018, 13, 1741
DOI: 10.1021/acschembio.7b01009

Logic-gated fluorescent biosensor integrating aptamer recognition and oxidative cleavage-responsive DNA circuit for myeloperoxidase detection

Bo-Yu Shi, Jia-Mei Zhang, Si-Han Qin, Han-Ye Yuan and Jing Wang

J Transl Med, 2026, 24
DOI: 10.1186/s12967-026-07780-4

Novel Peptide-Based Fluorescent Probe for Simultaneous Sensing of Chymotrypsin and Hydrogen Peroxide

David Milićević and Jan Hlaváč

ACS Omega, 2024
DOI: 10.1021/acsomega.4c00303

A Charge‐Switchable Zwitterionic Peptide for Rapid Detection of SARS‐CoV‐2 Main Protease

Zhicheng Jin, Yash Mantri, Maurice Retout, Yong Cheng, Jiajing Zhou, Alec Jorns, Pavla Fajtova, Wonjun Yim, Colman Moore, Ming Xu, Matthew N. Creyer, Raina M. Borum, Jingcheng Zhou, Zhuohong Wu, Tengyu He, William F. Penny, Anthony J. O'Donoghue and Jesse V. Jokerst

Angewandte Chemie, 2022, 134
DOI: 10.1002/ange.202112995

Process Analytical Technology Implementation for Peptide Manufacturing: Cleavage Reaction of Recombinant Lethal Toxin Neutralizing Factor Concatemer as a Case Study

Vishwanath Hebbi, Devendra Kumar and Anurag S. Rathore

Anal. Chem., 2020, 92, 5676
DOI: 10.1021/acs.analchem.9b05273

Efficiency Improvements and Discovery of New Substrates for a SARS-CoV-2 Main Protease FRET Assay

Tonko Dražić, Nikos Kühl, Mila M. Leuthold, Mira A.M. Behnam and Christian D. Klein

SLAS Discovery, 2021, 26, 1189
DOI: 10.1177/24725552211020681

A signal-on electrochemical biosensor for evaluation of caspase-3 activity and cell apoptosis by the generation of molecular electrocatalysts on graphene electrode surface for water oxidation

Dehua Deng, Yuanqiang Hao, Suling Yang, Qiuyang Han, Lin Liu, Yanrong Xiang, Fanbin Tu and Ning Xia

Sensors and Actuators B: Chemical, 2019, 286, 415
DOI: 10.1016/j.snb.2019.01.137

Protease Substrate-Independent Universal Assay for Monitoring Digestion of Native Unmodified Proteins

Emmiliisa Vuorinen, Salla Valtonen, Nazia Hassan, Randa Mahran, Huda Habib, Morteza Malakoutikhah, Kari Kopra and Harri Härmä

IJMS, 2021, 22, 6362
DOI: 10.3390/ijms22126362

Electrochemical protease detection strategies based on proteolytic cleavage

Seonhwa Park and Haesik Yang

Bulletin Korean Chem Soc, 2025, 46, 994
DOI: 10.1002/bkcs.70071

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

Ning Xia, Zhifang Sun, Fangyuan Ding, Yanan Wang, Wenna Sun and Lin Liu

ACS Sens., 2021, 6, 1166
DOI: 10.1021/acssensors.0c02415

AL-PHA beads: Bioplastic-based protease biosensors for global health applications

Richard J.R. Kelwick, Alexander J. Webb, Yizhou Wang, Amelie Heliot, Fiona Allan, Aidan M. Emery, Michael R. Templeton and Paul S. Freemont

Materials Today, 2021, 47, 25
DOI: 10.1016/j.mattod.2021.02.018

NIR-Sensitive Squaraine Dye—Peptide Conjugate for Trypsin Fluorogenic Detection

Priyanka Balyan, Shekhar Gupta, Sai Kiran Mavileti, Shyam S. Pandey and Tamaki Kato

Biosensors, 2024, 14, 458
DOI: 10.3390/bios14100458

Mitigating dithiothreitol interference to gold/thiol interface in electrochemical detection of cathepsin B activity toward multiplex protease analysis

Bingun Habarakadage, Sabari Rajendran, Zhaoyang Ren, Morgan J. Anderson, Jessica Koehne, Lingaraju Gorla, Shunya Morita, Sara Wu, Duy H. Hua and Jun Li

Biosensors and Bioelectronics, 2025, 273, 117193
DOI: 10.1016/j.bios.2025.117193

Protease functional assay on membrane

Garima Goyal, Alagappan Palaniappan and Bo Liedberg

Sensors and Actuators B: Chemical, 2020, 305, 127442
DOI: 10.1016/j.snb.2019.127442

Synthetic protein protease sensor platform

Ciaran Devoy, Yensi Flores Bueso, Stephen Buckley, Sidney Walker and Mark Tangney

Front. Bioeng. Biotechnol., 2024, 12
DOI: 10.3389/fbioe.2024.1347953

Washing- and Separation-Free Electrochemical Detection of Porphyromonas gingivalis in Saliva for Initial Diagnosis of Periodontitis

Seonhwa Park, Kiryeon Park, Hee Sam Na, Jin Chung and Haesik Yang

Anal. Chem., 2021, 93, 5644
DOI: 10.1021/acs.analchem.1c00572

Peptide-based electrochemical biosensors utilized for protein detection

Veronika Vanova, Katerina Mitrevska, Vedran Milosavljevic, David Hynek, Lukas Richtera and Vojtech Adam

Biosensors and Bioelectronics, 2021, 180, 113087
DOI: 10.1016/j.bios.2021.113087

Impedimetric biosensor for assay of caspase-3 activity and evaluation of cell apoptosis using self-assembled biotin-phenylalanine network as signal enhancer

Ning Xia, Yaliang Huang, Zhiyue Cui, Shudi Liu, Dehua Deng, Lin Liu and Jianxiu Wang

Sensors and Actuators B: Chemical, 2020, 320, 128436
DOI: 10.1016/j.snb.2020.128436

Development of a Bio-Layer Interferometry-Based Protease Assay Using HIV-1 Protease as a Model

Márió Miczi, Ádám Diós, Beáta Bozóki, József Tőzsér and János András Mótyán

Viruses, 2021, 13, 1183
DOI: 10.3390/v13061183

Next Generation of Fluorometric Protease Assays: 7‐Nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl‐amides (NBD‐Amides) as Class‐Spanning Protease Substrates

Hannah Maus, Patrick Müller, Mergim Meta, Sabrina N. Hoba, Stefan J. Hammerschmidt, Robert A. Zimmermann, Collin Zimmer, Natalie Fuchs, Tanja Schirmeister and Fabian Barthels

Chemistry A European J, 2023, 29
DOI: 10.1002/chem.202301855

Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures

G. Göbel, F. Müller, A. Talke, U. Ahnert and F. Lisdat

Bioelectrochemistry, 2024, 160, 108775
DOI: 10.1016/j.bioelechem.2024.108775

Surface Plasmon Resonance for Protease Detection by Integration of Homogeneous Reaction

Ning Xia, Gang Liu and Xinyao Yi

Biosensors, 2021, 11, 362
DOI: 10.3390/bios11100362

A fluorometric assay of thrombin using magnetic nanoparticles and enzyme-free hybridization chain reaction

Zhongming Huang, Dinggeng He and Hung-Wing Li

Microchim Acta, 2020, 187
DOI: 10.1007/s00604-020-04279-7

Overview on peptide-based electrochemical biosensors

Gang Liu, Yuanyuan Li, Meiling Liu, Jiayou Cheng, Suling Yang, Fengli Gao and Lin Liu

International Journal of Electrochemical Science, 2023, 18, 100395
DOI: 10.1016/j.ijoes.2023.100395

Continuous protease assays using liquid crystal as a reporter

Mahbuba Jannat and Kun-Lin Yang

Sensors and Actuators B: Chemical, 2018, 269, 8
DOI: 10.1016/j.snb.2018.04.125

A GMR enzymatic assay for quantifying nuclease and peptidase activity

Michael Sveiven, Ana K. Serrano, Joshua Rosenberg, Douglas J. Conrad, Drew A. Hall and Anthony J. O’Donoghue

Front. Bioeng. Biotechnol., 2024, 12
DOI: 10.3389/fbioe.2024.1363186

Electrochemical biosensors for the detection of matrix metalloproteinases

Jianlin Zhou

International Journal of Electrochemical Science, 2022, 17, 221034
DOI: 10.20964/2022.10.17

1,8-Naphthalimide based fluorescent sensors for enzymes

Conor Geraghty, Conor Wynne and Robert B.P. Elmes

Coordination Chemistry Reviews, 2021, 437, 213713
DOI: 10.1016/j.ccr.2020.213713

A colorimetric strategy for assay of protease activity based on gold nanoparticle growth controlled by ascorbic acid and Cu(II)-coordinated peptide

Lin Liu, Dehua Deng, Yiru Wang, Kewei Song, Ziling Shang, Qiao Wang, Ning Xia and Bing Zhang

Sensors and Actuators B: Chemical, 2018, 266, 246
DOI: 10.1016/j.snb.2018.03.116

Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference

Zhouxiang Ji, Xinqi Kang, Shaoying Wang and Peixuan Guo

Biomaterials, 2018, 182, 227
DOI: 10.1016/j.biomaterials.2018.08.005

Recent developments in capillary and microchip electroseparations of peptides (2017–mid 2019)

Václav Kašička

Electrophoresis, 2020, 41, 10
DOI: 10.1002/elps.201900269

Quantum/carbon dots-based fluorescent assays for enzyme activity

Xia Tong, Shuyun Shi, Chaoying Tong, Ali Iftikhar, Ruiqing Long and Yongfeng Zhu

TrAC Trends in Analytical Chemistry, 2020, 131, 116008
DOI: 10.1016/j.trac.2020.116008

Ultrasensitive Determination of Trypsin in Human Urine Based on Amplified Fluorescence Response

Taemin Park, Minwoo Han, Kirk S. Schanze and Seoung Ho Lee

ACS Sens., 2023, 8, 2591
DOI: 10.1021/acssensors.3c00297

Transducing Protease Activity into DNA Output for Developing Smart Bionanosensors

Hieu Bui, Carl W. Brown, Susan Buckhout‐White, Sebastián A. Díaz, Michael H. Stewart, Kimihiro Susumu, Eunkeu Oh, Mario G. Ancona, Ellen R. Goldman and Igor L. Medintz

Small, 2019, 15
DOI: 10.1002/smll.201805384

Detection of gingipain activity using solid state nanopore sensors

Quentin Palomar, Anna Svärd, Shuangshuang Zeng, Qitao Hu, Funing Liu, Daniel Aili and Zhen Zhang

Sensors and Actuators B: Chemical, 2022, 368, 132209
DOI: 10.1016/j.snb.2022.132209

Fluorescence-Based On-Resin Detection of Three Model Proteases

David Milićević and Jan Hlaváč

Chemosensors, 2021, 9, 359
DOI: 10.3390/chemosensors9120359

Influences of extracellular polymeric substances (EPS) recovered from waste sludge on the ability of Jiaozhou Bay to self-remediate of diesel-polluted seawater

Long Meng, Wen Li, Lanmei Zhao, Huaxiao Yan and Hui Zhao

Journal of Environmental Management, 2024, 353, 120196
DOI: 10.1016/j.jenvman.2024.120196

Highly Stable Passive Wireless Sensor for Protease Activity Based on Fatty Acid-Coupled Gelatin Composite Films

Palraj Kalimuthu, Juan F. Gonzalez-Martinez, Tautgirdas Ruzgas and Javier Sotres

Anal. Chem., 2020, 92, 13110
DOI: 10.1021/acs.analchem.0c02153