Issue 1, 2018

Micro free flow electrophoresis

Abstract

Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.

Graphical abstract: Micro free flow electrophoresis

Article information

Article type
Critical Review
Submitted
19 6 2017
Accepted
16 10 2017
First published
17 10 2017

Lab Chip, 2018,18, 27-40

Micro free flow electrophoresis

A. C. Johnson and M. T. Bowser, Lab Chip, 2018, 18, 27 DOI: 10.1039/C7LC01105A

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