Issue 45, 2017

Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

Abstract

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

Graphical abstract: Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

Supplementary files

Article information

Article type
Communication
Submitted
14 12 2016
Accepted
08 5 2017
First published
09 5 2017

Chem. Commun., 2017,53, 6109-6112

Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

J. A. González-Vera, D. Bouzada, C. Bouclier, M. Eugenio Vázquez and M. C. Morris, Chem. Commun., 2017, 53, 6109 DOI: 10.1039/C6CC09948C

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