Issue 25, 2023

Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

Abstract

Multiplex technology is an important emerging field, in diagnostic sciences, that enables the simultaneous detection of several analytes in a single sample. The light-emission spectrum of a chemiluminescent phenoxy-dioxetane luminophore can be accurately predicted by determining the fluorescence-emission spectrum of its corresponding benzoate species, which is generated during the chemiexcitation process. Based on this observation, we designed a library of chemiluminescent dioxetane luminophores with multicolor emission wavelengths. Two dioxetane luminophores that have different emission spectra, but similar quantum yield properties, were selected from the synthesized library for a duplex analysis. The selected dioxetane luminophores were equipped with two different enzymatic substrates to generate turn-ON chemiluminescent probes. This pair of probes exhibited a promising ability to act as a chemiluminescent duplex system for the simultaneous detection of two different enzymatic activities in a physiological solution. In addition, the pair of probes were also able to simultaneously detect the activities of the two enzymes in a bacterial assay, using a blue filter slit for one enzyme and a red filter slit for the other enzyme. As far as we know, this is the first successful demonstration of a chemiluminescent duplex system composed of two-color phenoxy-1,2-dioxetane luminophores. We believe that the library of dioxetanes presented here will be beneficial for developing chemiluminescence luminophores for multiplex analysis of enzymes and bioanalytes.

Graphical abstract: Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

Supplementary files

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Article information

Article type
Edge Article
Submitted
10 5 2023
Accepted
26 5 2023
First published
01 6 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 6953-6962

Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

S. Gutkin, R. Tannous, Q. Jaber, M. Fridman and D. Shabat, Chem. Sci., 2023, 14, 6953 DOI: 10.1039/D3SC02386A

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