Issue 4, 2023

The enhancement of enzyme cascading via tetrahedral DNA framework modification

Abstract

Enzyme clustering is widely used in many organisms to increase the catalytic efficiency of cascade reactions. Inspired by nature, organizing enzymes within a cascade reaction also draws much attention in both basic research and industrial processes. An important step for organizing enzymes precisely in vitro is enzyme modification. However, modifying enzymes without sacrificing their activity remains challenging until now. For example, labeling enzymes with DNA, one of the well-established enzyme modification methods, has been shown to significantly reduce the enzymatic activity. Herein we report an enzyme conjugation method that can rescue the reduction of enzymatic activity caused by DNA labeling. We demonstrate that immobilizing DNA-modified enzymes on the vertex of TDNs (tetrahedral DNA nanostructures) enhances the enzymatic activity compared with their unmodified counterparts. Using this strategy, we have further developed an ultra-sensitive and high-throughput electrochemical biosensor for sarcosine detection, which holds great promise for prostate cancer screening.

Graphical abstract: The enhancement of enzyme cascading via tetrahedral DNA framework modification

Supplementary files

Article information

Article type
Paper
Submitted
30 12 2022
Accepted
16 1 2023
First published
16 1 2023

Analyst, 2023,148, 906-911

The enhancement of enzyme cascading via tetrahedral DNA framework modification

H. Zhao, M. Li, S. Lu, N. Cao, X. Zuo, S. Wang and M. Li, Analyst, 2023, 148, 906 DOI: 10.1039/D2AN02097A

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