Issue 31, 2022

The rippled β-sheet layer configuration—a novel supramolecular architecture based on predictions by Pauling and Corey

Abstract

The rippled β-sheet is a peptidic structural motif related to but distinct from the pleated β-sheet. Both motifs were predicted in the 1950s by Pauling and Corey. The pleated β-sheet was since observed in countless proteins and peptides and is considered common textbook knowledge. Conversely, the rippled β-sheet only gained a meaningful experimental foundation in the past decade, and the first crystal structural study of rippled β-sheets was published as recently as this year. Noteworthy, the crystallized assembly stopped at the rippled β-dimer stage. It did not form the extended, periodic rippled β-sheet layer topography hypothesized by Pauling and Corey, thus calling the validity of their prediction into question. NMR work conducted since moreover shows that certain model peptides rather form pleated and not rippled β-sheets in solution. To determine whether the periodic rippled β-sheet layer configuration is viable, the field urgently needs crystal structures. Here we report on crystal structures of two racemic and one quasi-racemic aggregating peptide systems, all of which yield periodic rippled antiparallel β-sheet layers that are in excellent agreement with the predictions by Pauling and Corey. Our study establishes the rippled β-sheet layer configuration as a motif with general features and opens the road to structure-based design of unique supramolecular architectures.

Graphical abstract: The rippled β-sheet layer configuration—a novel supramolecular architecture based on predictions by Pauling and Corey

Supplementary files

Article information

Article type
Edge Article
Submitted
05 5 2022
Accepted
15 7 2022
First published
15 7 2022
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2022,13, 8947-8952

The rippled β-sheet layer configuration—a novel supramolecular architecture based on predictions by Pauling and Corey

A. Hazari, M. R. Sawaya, N. Vlahakis, T. C. Johnstone, D. Boyer, J. Rodriguez, D. Eisenberg and J. A. Raskatov, Chem. Sci., 2022, 13, 8947 DOI: 10.1039/D2SC02531K

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