Issue 93, 2022

Mass photometry reveals SARS-CoV-2 spike stabilisation to impede ACE2 binding through altered conformational dynamics

Abstract

Here we show using mass photometry how proline substitutions, commonly used for SARS-CoV-2 spike stabilisation in vaccine design, directly affects ACE2 receptor interactions via dynamics of open and closed states. Conformational changes and ACE2 binding were influenced by spike variant and temperature, but independent of site-specific N-glycosylation.

Graphical abstract: Mass photometry reveals SARS-CoV-2 spike stabilisation to impede ACE2 binding through altered conformational dynamics

Supplementary files

Article information

Article type
Communication
Submitted
25 8 2022
Accepted
24 10 2022
First published
28 10 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 12939-12942

Mass photometry reveals SARS-CoV-2 spike stabilisation to impede ACE2 binding through altered conformational dynamics

S. A. Burnap and W. B. Struwe, Chem. Commun., 2022, 58, 12939 DOI: 10.1039/D2CC04711J

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