Issue 76, 2022

A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells

Abstract

Aryl boronate fluorescent probes allow the non-invasive study of dynamic cellular processes involving the reactive species, hydrogen peroxide (H2O2) and peroxynitrite (ONOO). However, the ability of these probes to differentiate between these two species remains unclear. Here, we report a boronate-functionalised hemicyanine dye (HD-BPin) as a potential strategy to distinguish between H2O2 at 704 nm (red channel) and ONOO at 460 nm (blue channel) in solution and in cells. This work also highlights the choice of fluorophore before boronate functionalization can dictate the observed selectivity between these two species.

Graphical abstract: A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells

Supplementary files

Article information

Article type
Communication
Submitted
17 6 2022
Accepted
30 8 2022
First published
30 8 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 10699-10702

A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells

H. R. Bolland, E. M. Hammond and A. C. Sedgwick, Chem. Commun., 2022, 58, 10699 DOI: 10.1039/D2CC03406A

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