Issue 48, 2022

Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Abstract

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

Graphical abstract: Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Supplementary files

Article information

Article type
Communication
Submitted
12 4 2022
Accepted
19 5 2022
First published
24 5 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 6857-6860

Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Y. Du, R. C. May and H. J. Cooper, Chem. Commun., 2022, 58, 6857 DOI: 10.1039/D2CC02085H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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