Issue 22, 2022

A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors

Abstract

Glutathione (GSH) is known to play a key role in the modulation of the redox environment in N-methyl-d-aspartate (NMDA) receptors. Coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties was synthesized and reported to monitor GSH near NMDA receptors. The cyanoacrylamide moiety allows probe 1 to monitor GSH reversibly at pH 7.4 and the ifenprodil group acts as a directing group for NMDA receptors. Two-photon fluorescence microscopy allows probe 1 to successfully sense endogenous GSH in neuronal cells and hippocampal tissues with excitation at 750 nm. Furthermore, the addition of H2O2 and GSH induced a decrease and an increase in fluorescence emission. Probe 1 can serve as a potential practical imaging tool to get important information on GSH in the brain.

Graphical abstract: A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors

Supplementary files

Article information

Article type
Communication
Submitted
30 9 2021
Accepted
16 2 2022
First published
24 2 2022

Chem. Commun., 2022,58, 3633-3636

A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-D-aspartate (NMDA) receptors

N. Kwon, C. S. Lim, D. Lee, G. Ko, J. Ha, M. Cho, K. M. K. Swamy, E. Lee, D. J. Lee, S. Nam, X. Zhou, H. M. Kim and J. Yoon, Chem. Commun., 2022, 58, 3633 DOI: 10.1039/D1CC05512G

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