Issue 6, 2022

Paving the way towards continuous biosensing by implementing affinity-based nanoswitches on state-dependent readout platforms

Abstract

Continuous biosensors provide real-time information about biochemical processes occurring in the environment of interest and are therefore highly desirable in research, diagnostics and industrial settings. Although remarkable progress has been made in the field of biosensing, most biosensors still rely on batch processes and, thus, are not suited to perform continuous measurements. Recently, however, it has been shown that by combining affinity-based nanoswitches with state-dependent readout platforms, the necessity for batch processes can be overcome and affinity-based continuous biosensing can be achieved. In this review, we first provide an overview of affinity-based continuous biosensing and discuss the required components to achieve this goal. More specifically, we summarize the strategies that have been applied to develop and tune both protein and nucleic acid-based switches, as well as readout strategies that can be applied in combination with the former. Afterwards, biosensors in which both elements were already integrated and hence enabled continuous measurements are reviewed. We also discuss the challenges and opportunities associated with each approach and therefore believe this review can help to encourage and guide future research towards continuous biosensing.

Graphical abstract: Paving the way towards continuous biosensing by implementing affinity-based nanoswitches on state-dependent readout platforms

Article information

Article type
Critical Review
Submitted
22 12 2021
Accepted
15 2 2022
First published
15 2 2022

Analyst, 2022,147, 1006-1023

Paving the way towards continuous biosensing by implementing affinity-based nanoswitches on state-dependent readout platforms

A. Dillen and J. Lammertyn, Analyst, 2022, 147, 1006 DOI: 10.1039/D1AN02308J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements