Issue 6, 2021

Development of the C12Im-Cl-assisted method for rapid sample preparation in proteomic application

Abstract

Chromatography and mass spectrometry (MS) techniques have greatly improved the power of proteomic analyses. However, sample processing methods used prior to MS, including protein extraction and digestion, remain bottlenecks in the large-scale clinical application of proteomics. Ionic liquids, composed entirely of ions, have high solubility in various solvents. In this study, the effects of the cationic surfactant 1-dodecyl-3-methylimidazolium chloride (C12Im-Cl) on protein digestion were evaluated for clinical proteomic applications. C12Im-Cl was compatible with trypsin and reduced the protein digestion time from 16 h to 1 h. Residual C12Im-Cl was easily removed with a strong anion exchange membrane before MS. We evaluated the performance of C12Im-Cl extraction and rapid protein digestion using formalin-fixed paraffin-embedded liver cancer tissues. The number of proteins and peptides identified was nearly equal to that identified by the traditional filter-aided sample preparation method (2705 vs. 2739 and 16 682 vs. 17 214). In general, the C12Im-Cl-aided rapid sample preparation method is promising for proteomic applications.

Graphical abstract: Development of the C12Im-Cl-assisted method for rapid sample preparation in proteomic application

Supplementary files

Article information

Article type
Paper
Submitted
08 11 2020
Accepted
29 12 2020
First published
04 1 2021

Anal. Methods, 2021,13, 776-781

Development of the C12Im-Cl-assisted method for rapid sample preparation in proteomic application

C. Liu, X. Si, S. Yan, X. Zhao, X. Qian, W. Ying and L. Zhao, Anal. Methods, 2021, 13, 776 DOI: 10.1039/D0AY02079F

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