Issue 48, 2020

A single amino acid Gly-tag enables metal-free protein purification

Abstract

Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C–C bond dissociation by resonance-assisted electron density polarization.

Graphical abstract: A single amino acid Gly-tag enables metal-free protein purification

Supplementary files

Article information

Article type
Edge Article
Submitted
26 2 2020
Accepted
26 10 2020
First published
26 10 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2020,11, 13137-13142

A single amino acid Gly-tag enables metal-free protein purification

L. Purushottam, U. V. B., D. G. Rawale, M. Gujrati, S. D. Mishra, S. T. K., N. C. Reddy, S. R. Adusumalli, R. K. Mishra and V. Rai, Chem. Sci., 2020, 11, 13137 DOI: 10.1039/D0SC01153C

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