Issue 32, 2020

Biodegradable microneedle patch for transdermal gene delivery

Abstract

The skin houses a developed vascular and lymphatic network with a significant population of immune cells. Because of the properties of the skin, nucleic acid delivery through the tissue has the potential to treat a range of pathologies, including genetic skin conditions, hyperproliferative diseases, cutaneous cancers, wounds, and infections. This work presents a gelatin methacryloyl (GelMA) microneedle (MN)-based platform for local and controlled transdermal delivery of plasmid DNA (pDNA) with high transfection efficiency both in vitro and in vivo. Intracellular delivery of the nucleic acid cargo is enabled by poly(β-amino ester) (PBAE) nanoparticles (NPs). After being embedded in the GelMA MNs, sustained release of DNA-encapsulated PBAE NPs is achieved and the release profiles can be controlled by adjusting the degree of crosslinking of the GelMA hydrogel. These results highlight the advantages and potential of using PBAE/DNA NP-embedded GelMA MN patches (MN/PBAE/DNA) for successful transdermal delivery of pDNA for tissue regeneration and cancer therapy.

Graphical abstract: Biodegradable microneedle patch for transdermal gene delivery

Supplementary files

Article information

Article type
Communication
Submitted
07 4 2020
Accepted
23 7 2020
First published
12 8 2020

Nanoscale, 2020,12, 16724-16729

Biodegradable microneedle patch for transdermal gene delivery

M. Qu, H. Kim, X. Zhou, C. Wang, X. Jiang, J. Zhu, Y. Xue, P. Tebon, S. A. Sarabi, S. Ahadian, M. R. Dokmeci, S. Zhu, Z. Gu, W. Sun and A. Khademhosseini, Nanoscale, 2020, 12, 16724 DOI: 10.1039/D0NR02759F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements