Issue 98, 2020

Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

Abstract

The bottom-up construction of bio-inspired systems capable of self-maintenance and reproduction is a central goal in systems chemistry and synthetic biology. A particular challenge in such systems is the continuous regeneration of key proteins required for macromolecular synthesis. Here, we probe self-maintenance of a reconstituted in vitro translation system challenged by serial transfer of selected key proteins. We find that the system can simultaneously regenerate multiple essential polypeptides, which then contribute to the maintenance of protein expression after serial transfer. The presented strategy offers a robust methodology for probing and optimizing continuous self-regeneration of proteins in cell-free environments.

Graphical abstract: Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

Supplementary files

Article information

Article type
Communication
Submitted
28 9 2020
Accepted
18 11 2020
First published
19 11 2020
This article is Open Access
Creative Commons BY license

Chem. Commun., 2020,56, 15426-15429

Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer

K. Libicher and H. Mutschler, Chem. Commun., 2020, 56, 15426 DOI: 10.1039/D0CC06515C

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