Issue 2, 2019

A self-assembling peptide hydrogel for ultrarapid 3D bioassays

Abstract

Biosensing analytical platforms rely on the intimate structure–function relationship of immobilized probes. In this context, hydrogels are appealing semi-wet systems to locally confine biomolecules while preserving their structural integrity and function. Yet, limitations imposed by biomolecule diffusion rates or fabrication difficulties still hamper their broad application. Here, using a self-assembling peptide, a printable and self-adhesive hydrogel was obtained and applied to fabricate arrays of localized bio-functional 3D microenvironments on analytical interfaces. This soft matrix represents a robust and versatile material, allowing fast and selective tuning of analyte diffusion, which is exploited here to run in-gel immunoassays under solution-like conditions in an unprecedented (<10 min) time frame. The developed material overcomes major limitations associated with hydrogels for bioassays, widening the prospects for easy fabrication of multifunctional bio-interfaces for high-throughput, molecular recognition assays.

Graphical abstract: A self-assembling peptide hydrogel for ultrarapid 3D bioassays

Supplementary files

Article information

Article type
Communication
Submitted
22 8 2018
Accepted
22 10 2018
First published
22 10 2018
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2019,1, 490-497

A self-assembling peptide hydrogel for ultrarapid 3D bioassays

P. Gagni, A. Romanato, G. Bergamaschi, P. Bettotti, R. Vanna, C. Piotto, C. F. Morasso, M. Chiari, M. Cretich and A. Gori, Nanoscale Adv., 2019, 1, 490 DOI: 10.1039/C8NA00158H

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