Issue 24, 2017

An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range

Abstract

Although multi-point, multi-color fluorescence-detection systems are widely used in various sciences, they would find wider applications if they are miniaturized. Accordingly, an ultra-small, four-emission-point and four-color fluorescence-detection system was developed. Its size (space between emission points and a detection plane) is 15 × 10 × 12 mm, which is three-orders-of-magnitude smaller than that of a conventional system. Fluorescence from four emission points with an interval of 1 mm on the same plane was respectively collimated by four lenses and split into four color fluxes by four dichroic mirrors. Then, a total of sixteen parallel color fluxes were directly input into an image sensor and simultaneously detected. The emission-point plane and the detection plane (the image-sensor surface) were parallel and separated by a distance of only 12 mm. The developed system was applied to four-capillary array electrophoresis and successfully achieved Sanger DNA sequencing. Moreover, compared with a conventional system, the developed system had equivalent high fluorescence-detection sensitivity (lower detection limit of 17 pM dROX) and 1.6-orders-of-magnitude higher dynamic range (4.3 orders of magnitude).

Graphical abstract: An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range

Supplementary files

Article information

Article type
Paper
Submitted
04 10 2017
Accepted
23 10 2017
First published
08 11 2017
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2017,17, 4231-4242

An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range

T. Anazawa and M. Yamazaki, Lab Chip, 2017, 17, 4231 DOI: 10.1039/C7LC01070B

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